Serotyping of Cryptococcus neoformans Strains Isolated from Clinical Specimens in Thailand and Their Susceptibility to Various Antifungal Agents

One hundred and thirty-nine strains of Cryptococcus neoformans were isolated in Thailand from clinical specimens including 97 AIDS patients: 67 from Northern, 48 from Central, 17 from Northeastern and 7 from Southern regional hospitals. Six out of the 139 strains were serotype B and the remaining 13...

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Bibliographic Details
Published in:European journal of epidemiology 1997-04, Vol.13 (3), p.335-340
Main Authors: Poonwan, Natteewan, Mikami, Yuzuru, Poosuwan, Suwan, Boon-Long, Jotica, Mekha, Nanthawan, Kusum, Mayura, Yazawa, Katsukiyo, Tanaka, Reiko, Nishimura, Kazuko, Konyama, Kazuichi
Format: Article
Language:English
Subjects:
Acquired immune deficiency syndrome
AIDS
AIDS-Related Opportunistic Infections - microbiology
AIDS/HIV
Antifungal agents
Antifungal Agents - pharmacology
Antifungals
Biological and medical sciences
Cerebrospinal Fluid - microbiology
Chemotherapy
Cryptococcosis
Cryptococcosis - microbiology
Cryptococcus neoformans
Cryptococcus neoformans - drug effects
Cryptococcus neoformans - isolation & purification
Fungal infections
Fungi
Genetic polymorphism
Genotypes
HIV
Human mycoses
Humans
Infectious diseases
Medical sciences
Microbial Sensitivity Tests
Miscellaneous mycoses
Mycoses
Polymerase chain reaction
Serotyping
Thailand
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Summary:One hundred and thirty-nine strains of Cryptococcus neoformans were isolated in Thailand from clinical specimens including 97 AIDS patients: 67 from Northern, 48 from Central, 17 from Northeastern and 7 from Southern regional hospitals. Six out of the 139 strains were serotype B and the remaining 133 were A. There was no correlation between serotypes and regional distribution. To our knowledge, this is the first report of serotyping studies on C. neoformans in Thailand. Studies on random amplified polymorphic DNA (RAPD) analysis showed that this method is useful for the differentiation of C. neoformans var. gattii (serotypes B and C) and C. neoformans var. neoformans (serotypes A and D). They also indicated that Thai isolates of C. neoformans var. gattii (serotype B) were a homogeneous group on the basis of their genotypes. Antifungal susceptibility tests using 5 antifungal agents including amphotericin B, fluconazole, flucytosine, itraconazole and micronazole against 50 selected strains of C. neoformans showed that they were sensitive to all of the antifungal agents tested except for one strain that was resistant to flucytosine.
ISSN:0393-2990
1573-7284
DOI:10.1023/A:1007376917836