Rat Kidney Thromboxane Synthase: cDNA Cloning and Gene Expression Regulation in Hydronephrotic Kidney

We isolated a rat homolog of thromboxane (TX) synthase cDNA (∼ 1.8 kb) from the kidney with a fragment of human TX synthase cDNA amplified by polymerase chain reaction with placenta cDNA as a template. Northern blot analysis has shown that rat TX synthase gene is expressed abundantly in lung, liver,...

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Bibliographic Details
Published in:Prostaglandins 1997-06, Vol.53 (6), p.423-431
Main Authors: Tsutsumi, Eikatsu, Takeuchi, Kazuhisa, Abe, Takaaki, Takahashi, Nobuyuki, Kato, Taro, Taniyama, Yoshihiro, Ikeda, Yukio, Ito, Sadayoshi, Abe, Keishi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Cloning, Molecular
DNA, Complementary - genetics
Female
gene expression
Gene Expression Regulation
Humans
hydronephrosis
Hydronephrosis - enzymology
Kidney - enzymology
Molecular Sequence Data
Placenta - chemistry
Polymerase Chain Reaction
prostaglandin
Rats
Rats, Sprague-Dawley
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
Thromboxane-A Synthase - chemistry
Thromboxane-A Synthase - genetics
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Summary:We isolated a rat homolog of thromboxane (TX) synthase cDNA (∼ 1.8 kb) from the kidney with a fragment of human TX synthase cDNA amplified by polymerase chain reaction with placenta cDNA as a template. Northern blot analysis has shown that rat TX synthase gene is expressed abundantly in lung, liver, and uterus; moderately in kidney. TX synthase mRNA expression was up-regulated in hydronephrotic kidney made by ureter ligation. In conclusion, we have revealed the structure of rat kidney TX synthase. Up-regulation of renal TX synthase, which may cause stimulation of TX synthesis, is possibly implicated in the tissue injury in hydronephrotic kidney.
ISSN:0090-6980
DOI:10.1016/S0090-6980(97)00059-2