Modulation of Transcription Factor Sp1 by cAMP-dependent Protein Kinase

Transcription factor Sp1 is a phosphoprotein whose level and DNA binding activity are markedly increased in doxorubicin-resistant HL-60 (HL-60/AR) leukemia cells. Thetrans-activating and DNA binding properties of Sp1 in HL-60/AR cells are stimulated by cAMP-dependent protein kinase (PKA) and PKA ago...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1997-08, Vol.272 (34), p.21137-21141
Main Authors: Rohlff, Christian, Ahmad, Shakeel, Borellini, Flavia, Lei, Jun, Glazer, Robert I.
Format: Article
Language:English
Subjects:
Cyclic AMP - physiology
Cyclic AMP-Dependent Protein Kinases - metabolism
DNA-Binding Proteins - metabolism
HL-60 Cells
Humans
Phosphorylation
Signal Transduction
simian virus 40
Sp1 Transcription Factor - metabolism
Transcription, Genetic
Transcriptional Activation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Transcription factor Sp1 is a phosphoprotein whose level and DNA binding activity are markedly increased in doxorubicin-resistant HL-60 (HL-60/AR) leukemia cells. Thetrans-activating and DNA binding properties of Sp1 in HL-60/AR cells are stimulated by cAMP-dependent protein kinase (PKA) and PKA agonists and inhibited by PKA antagonists as well as by the PKA regulatory subunit. Reporter gene activity under the control of the Sp1-dependent SV40 promoter is stimulated in insect cells transiently expressing Sp1 and PKA, and the DNA binding activity of recombinant Sp1 is activated by exogenous PKA in vitro. These results indicate that Sp1 is a cAMP-responsive transcription factor and that Sp1-dependent genes may be modulated through a cAMP-dependent signaling pathway.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.272.34.21137