Miniaturized HPLC and ionspray mass spectrometry applied to the analysis of Paclitaxel and taxanes

Analysis of the antitumor agent Paclitaxel, related taxane analogues and yew tree bark extracts has been carried out using an HPLC system capable of performing chromatographic separations with conventional, small-bore, and micro-bore columns. Both diode array detector and mass spectrometry were inco...

Full description

Saved in:
Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 1997-07, Vol.15 (11), p.1729-1739
Main Authors: Liu, Jinping, Volk, Kevin J, Mata, Michelle J, Kerns, Edward H, Lee, Mike S
Format: Article
Language:English
Subjects:
Analysis
Antineoplastic Agents, Phytogenic - analysis
Biological and medical sciences
Bridged-Ring Compounds - analysis
Chromatography, High Pressure Liquid
General pharmacology
Ionspray mass spectrometry
Mass Spectrometry
Medical sciences
Miniaturized HPLC
Paclitaxel
Paclitaxel - analysis
Pharmacology. Drug treatments
Taxanes
Taxoids
Yew tree bark extracts
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Analysis of the antitumor agent Paclitaxel, related taxane analogues and yew tree bark extracts has been carried out using an HPLC system capable of performing chromatographic separations with conventional, small-bore, and micro-bore columns. Both diode array detector and mass spectrometry were incorporated into this system, providing additional spectral and structural information for identification of unknown samples. In conjunction with some basic theoretical studies dealing with miniaturized HPLC systems, experiments were designed to minimize the contribution of extra-column variances. Three chromatographic columns, 4.6, 2, and 1 mm i.d., were evaluated using a standard mixture consisting of Paclitaxel and three analogues. The experimental results obtained in these columns demonstrated good correlation with theoretical calculations with respect to the sensitivity enhancement. Studies on the combination of miniaturized HPLC with ionspray mass spectrometry for Paclitaxel samples showed dramatic improvement of MS performance as compared to conventional LC/MS. The advantages of this miniaturized LC/MS system are evidenced by enhanced mass sensitivity, which was more than two order of magnitude higher when changed from a 4.6 mm i.d. column to a 2.0 mm i.d. column, greatly improved peak shape, and the potential gain of efficiency. These studies demonstrate great potential of miniaturized HPLC/MS systems for structural characterization and confirmation of various pharmaceutical compounds.
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(96)01969-3