Immunohistochemical processing of tissue which allows for embedding in Araldite®, and orientation and correlation between light and electron microscopic staining

This paper describes a simple and efficient method for the immmunohistochemical processing of tissue sections on glass slides and the subsequent transfer of the entire section to an Araldite® medium. These sections present in one plane can then be orientated and when cut provide the user with whole...

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Bibliographic Details
Published in:Journal of microscopy (Oxford) 1997-07, Vol.187 (1), p.68-72
Main Authors: Oates, P. S., Light, A., Thomas, C.
Format: Article
Language:English
Subjects:
Animals
Correlation
embedding
Epoxy Resins
examination
immunohistochemistry
Immunohistochemistry - methods
light microscopy
Male
Microscopy, Electron
orientation
Phthalic Anhydrides
Rats
Rats, Wistar
Staining and Labeling
TEM
tissue
Tissue Embedding
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Description
Summary:This paper describes a simple and efficient method for the immmunohistochemical processing of tissue sections on glass slides and the subsequent transfer of the entire section to an Araldite® medium. These sections present in one plane can then be orientated and when cut provide the user with whole tissue, free from gaps due to folding. This procedure therefore enables the changes in protein concentration and distribution to be assessed and correlated within and between different cells at the light and electron microscopic level. This procedure may be of use in other microscopic techniques studies.
ISSN:0022-2720
1365-2818
DOI:10.1046/j.1365-2818.1997.2110775.x