Heterologous biopharmaceutical protein expression in Streptomyces

The commercial production of human proteins in recombinant microorganisms for therapeutic use is well established. Systems have been developed to exploit the natural ability of certain bacteria to secrete properly folded, bioactive proteins into the extracellular medium. The streptomycetes are a rel...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 1997-08, Vol.15 (8), p.315-320
Main Authors: Binnie, Craig, Douglas Cossar, J., Stewart, Donald I.H.
Format: Article
Language:English
Subjects:
Bacterial Proteins - biosynthesis
Bacteriology
Biological and medical sciences
Biopharmaceutics - trends
Biotechnology
Fundamental and applied biological sciences. Psychology
Humans
Metabolism. Enzymes
Microbiology
Recombinant Proteins - biosynthesis
Streptomyces - metabolism
Streptomyces lividans
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Summary:The commercial production of human proteins in recombinant microorganisms for therapeutic use is well established. Systems have been developed to exploit the natural ability of certain bacteria to secrete properly folded, bioactive proteins into the extracellular medium. The streptomycetes are a relatively well-characterized group of nonpathogenic filamentous bacteria that have the capacity to secrete large amounts of protein. In particular, Streptomyces lividans has the ability to secrete human proteins at a commercially viable level, thanks to relatively wellestablished plasmid-based expression systems, a high-biomass fermentation process and a low level of endogenous protease activity.
ISSN:0167-7799
1879-3096
DOI:10.1016/S0167-7799(97)01062-7