A direct method for the chromosomal assignment of DNA markers in Leishmania

A simple method for the chromosomal assignment of any DNA marker would be an important tool for the ongoing project to map the genome of the protozoan parasite Leishmania. The Leishmania chromosomes enter pulsed field gel electrophoresis (PFGE) gels under current electrophoretic conditions, but thei...

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Bibliographic Details
Published in:Gene 1997-07, Vol.194 (1), p.77-80
Main Authors: Wincker, Patrick, Ravel, Christophe, Britto, Constança, Dubessay, Pascal, Bastien, Patrick, Pagès, Michel, Blaineau, Christine
Format: Article
Language:English
Subjects:
Animals
Chromosome Banding
Chromosome Mapping
DNA, Protozoan - genetics
Electrophoresis, Gel, Pulsed-Field
Genes, Protozoan
Genetic Markers
Genome
Karyotyping
Leishmania
Leishmania - classification
Leishmania - genetics
Leishmania genome map
Linkage groups
Parasite genome
Polymorphism, Genetic
Pulsed field gel electrophoresis
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Summary:A simple method for the chromosomal assignment of any DNA marker would be an important tool for the ongoing project to map the genome of the protozoan parasite Leishmania. The Leishmania chromosomes enter pulsed field gel electrophoresis (PFGE) gels under current electrophoretic conditions, but their direct identification in a given strain is hampered by their stacking in a few chromosomal bands, and by the very frequent size variations of the same chromosome among parasite strains. To overcome these problems, we determined the complete karyotypes of 12 Old World Leishmania cloned strains. This enabled us to select three of these strains that display great chromosome size polymorphisms, such that every chromosome can be individualized by a specific pattern after hybridization onto these three karyotypes. The complete resolution of the genomes of these three strains can be carried out with only three electrophoretic conditions. This makes a series of three blots sufficient for the assignment of any new marker on a particular Leishmania chromosome.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(97)00162-5