Ccmar1, a full-length mariner element from the Mediterranean fruit fly, Ceratitis capitata

Using a PCR primer specific to the ITR sequence of a deleted mariner element we amplified a fragment of approximately 1300 bp from the genome of Ceratitis capitata. Analysis of four clones showed that they differed by approximately 4.6% in nucleotide sequence and exhibited high homology to mariner e...

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Bibliographic Details
Published in:Insect molecular biology 1997-08, Vol.6 (3), p.241-253
Main Authors: Gomulski, L.M, Torti, C, Malacrida, A.R, Gasperi, G
Format: Article
Language:English
Subjects:
Amino Acid Sequence
amino acid sequences
Animals
Base Sequence
ccmar1 transposase
Ceratitis capitata
cloning
Cloning, Molecular
Consensus Sequence - genetics
Diptera - genetics
DNA Transposable Elements - genetics
Evolution, Molecular
genbank/u40493
genbank/u76903
genbank/u76904
genbank/u76905
Gene Dosage
Genetic Variation - genetics
genomics
Molecular Sequence Data
nucleotide sequences
Open Reading Frames - genetics
Phylogeny
repetitive sequences
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Tephritidae
terminal repeat sequences
transposons
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Summary:Using a PCR primer specific to the ITR sequence of a deleted mariner element we amplified a fragment of approximately 1300 bp from the genome of Ceratitis capitata. Analysis of four clones showed that they differed by approximately 4.6% in nucleotide sequence and exhibited high homology to mariner elements of the mellifera subfamily. One clone in particular, Ccmar1.18, was found to possess an ORF of 338 amino acids together with many of the features typical of mariner elements. The consensus sequence, Ccmar1, derived from these clones is presented. Maximum parsimony phylogenetic analysis of the Ccmar1 element confirms its position at the periphery of the mariner mellifera subfamily. The Ccmar1 element is estimated to be present in about 500 copies in the genome. The evolutionary history of the element in relation to the colonization history of the medfly is discussed.
ISSN:0962-1075
1365-2583
DOI:10.1046/j.1365-2583.1997.00179.x