white gene from the yellow fever mosquito, Aedes aegypti

We report the cloning and primary characterization of both cDNA and genomic fragments from the white gene of the yellow fever mosquito, Aedes aegypti. Comparisons of the conceptual translation product with white genes from four other species within the order Diptera show that the Ae. aegypti gene is...

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Bibliographic Details
Published in:Insect molecular biology 1997-08, Vol.6 (3), p.291-299
Main Authors: Coates, C.J, Schaub, T.L, Besansky, N.J, Collins, F.H, James, A.A
Format: Article
Language:English
Subjects:
Aedes - genetics
Aedes aegypti
Amino Acid Sequence
amino acid sequences
Animals
ATP-Binding Cassette Transporters
Base Sequence
cloning
Cloning, Molecular
complementary DNA
Culicidae
disease vectors
Drosophila Proteins
Eye Proteins - chemistry
Eye Proteins - genetics
Freshwater
genbank/u88851
genbank/u88852
Genes, Insect - genetics
Genetic Markers
introns
Introns - genetics
Molecular Sequence Data
nucleotide sequences
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
structural genes
Transformation, Genetic
yellow fever
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Summary:We report the cloning and primary characterization of both cDNA and genomic fragments from the white gene of the yellow fever mosquito, Aedes aegypti. Comparisons of the conceptual translation product with white genes from four other species within the order Diptera show that the Ae. aegypti gene is most similar to the white gene of the mosquito vector of human malaria, Anopheles gambiae (86% identity and 92% similarity). The analysis of the primary sequence of genomic DNA at the 5'-end of the coding region revealed the presence of an intron that is also present in An. gambiae, but not in the vinegar fly, Drosophila melanogaster. The isolated clones of the Ae. aegypti white gene will enable the construction of a marker gene for use in the development of a germline transformation system for this species.
ISSN:0962-1075
1365-2583
DOI:10.1046/j.1365-2583.1997.00183.x