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white gene from the yellow fever mosquito, Aedes aegypti

We report the cloning and primary characterization of both cDNA and genomic fragments from the white gene of the yellow fever mosquito, Aedes aegypti. Comparisons of the conceptual translation product with white genes from four other species within the order Diptera show that the Ae. aegypti gene is...

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Published in:	Insect molecular biology 1997-08, Vol.6 (3), p.291-299
Main Authors:	Coates, C.J, Schaub, T.L, Besansky, N.J, Collins, F.H, James, A.A
Format:	Article
Language:	English
Subjects:	Aedes - genetics Aedes aegypti Amino Acid Sequence amino acid sequences Animals ATP-Binding Cassette Transporters Base Sequence cloning Cloning, Molecular complementary DNA Culicidae disease vectors Drosophila Proteins Eye Proteins - chemistry Eye Proteins - genetics Freshwater genbank/u88851 genbank/u88852 Genes, Insect - genetics Genetic Markers introns Introns - genetics Molecular Sequence Data nucleotide sequences Sequence Alignment Sequence Analysis, DNA Sequence Homology, Nucleic Acid structural genes Transformation, Genetic yellow fever
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cited_by	cdi_FETCH-LOGICAL-c4563-f0a8ad1250146259b72474141ebbe97c3113a5cc2586cec0e1f0a8490a7e3da13
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container_title	Insect molecular biology
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creator	Coates, C.J Schaub, T.L Besansky, N.J Collins, F.H James, A.A
description	We report the cloning and primary characterization of both cDNA and genomic fragments from the white gene of the yellow fever mosquito, Aedes aegypti. Comparisons of the conceptual translation product with white genes from four other species within the order Diptera show that the Ae. aegypti gene is most similar to the white gene of the mosquito vector of human malaria, Anopheles gambiae (86% identity and 92% similarity). The analysis of the primary sequence of genomic DNA at the 5'-end of the coding region revealed the presence of an intron that is also present in An. gambiae, but not in the vinegar fly, Drosophila melanogaster. The isolated clones of the Ae. aegypti white gene will enable the construction of a marker gene for use in the development of a germline transformation system for this species.
doi_str_mv	10.1046/j.1365-2583.1997.00183.x
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Comparisons of the conceptual translation product with white genes from four other species within the order Diptera show that the Ae. aegypti gene is most similar to the white gene of the mosquito vector of human malaria, Anopheles gambiae (86% identity and 92% similarity). The analysis of the primary sequence of genomic DNA at the 5'-end of the coding region revealed the presence of an intron that is also present in An. gambiae, but not in the vinegar fly, Drosophila melanogaster. 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Comparisons of the conceptual translation product with white genes from four other species within the order Diptera show that the Ae. aegypti gene is most similar to the white gene of the mosquito vector of human malaria, Anopheles gambiae (86% identity and 92% similarity). The analysis of the primary sequence of genomic DNA at the 5'-end of the coding region revealed the presence of an intron that is also present in An. gambiae, but not in the vinegar fly, Drosophila melanogaster. 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Comparisons of the conceptual translation product with white genes from four other species within the order Diptera show that the Ae. aegypti gene is most similar to the white gene of the mosquito vector of human malaria, Anopheles gambiae (86% identity and 92% similarity). The analysis of the primary sequence of genomic DNA at the 5'-end of the coding region revealed the presence of an intron that is also present in An. gambiae, but not in the vinegar fly, Drosophila melanogaster. The isolated clones of the Ae. aegypti white gene will enable the construction of a marker gene for use in the development of a germline transformation system for this species.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>9272447</pmid><doi>10.1046/j.1365-2583.1997.00183.x</doi><tpages>9</tpages></addata></record>
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subjects	Aedes - genetics Aedes aegypti Amino Acid Sequence amino acid sequences Animals ATP-Binding Cassette Transporters Base Sequence cloning Cloning, Molecular complementary DNA Culicidae disease vectors Drosophila Proteins Eye Proteins - chemistry Eye Proteins - genetics Freshwater genbank/u88851 genbank/u88852 Genes, Insect - genetics Genetic Markers introns Introns - genetics Molecular Sequence Data nucleotide sequences Sequence Alignment Sequence Analysis, DNA Sequence Homology, Nucleic Acid structural genes Transformation, Genetic yellow fever
title	white gene from the yellow fever mosquito, Aedes aegypti
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