An Assay for the Quantitation of Photofrin in Tissues and Fluids

A method for determining the concentration of Photofrin in tissues and biological fluids was developed. The procedure is based on the dissolution of biological material with Solvable a commercially available tissue so‐lubilizer, followed by porphyrin‐specific fluorescence detection and measurement....

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Bibliographic Details
Published in:Photochemistry and photobiology 1997-08, Vol.66 (2), p.237-244
Main Authors: Bellnier, David A., Greco, William R., Parsons, John C., Oseroff, Allan R., Kuebler, Alex, Dougherty, Thomas J.
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents - analysis
Calibration
Dihematoporphyrin Ether - analysis
Mice
Reproducibility of Results
Solubility
Spectrometry, Fluorescence - methods
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Summary:A method for determining the concentration of Photofrin in tissues and biological fluids was developed. The procedure is based on the dissolution of biological material with Solvable a commercially available tissue so‐lubilizer, followed by porphyrin‐specific fluorescence detection and measurement. It was found necessary to use a quadratic standard curve for the estimation of unknown Photofrin concentrations. While this method is limited to compounds that are stable in strong base, it has the advantages of being sensitive, rapid and low cost.
ISSN:0031-8655
1751-1097
DOI:10.1111/j.1751-1097.1997.tb08649.x