Immune response and protection in mice inoculated with Leishmania amazonensis clones expressing different degrees of virulence

The induction of protective immunity to Leishmania amazonensis was investigated by injection of parasite clones of low and medium virulence into susceptible mice. To this end, L. amazonensis were cloned by limiting dilution and the clones' virulence was evaluated by the course of infection in s...

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Bibliographic Details
Published in:Parasitology research (1987) 1997, Vol.83 (7), p.690-697
Main Authors: SOARES DA FONSECA, A. L, LIMA VALLOCHI, A, DE CAMARGO FURTADO, G, DE ALMEIDA ABRAHAMSOHN, I, COLLET DE ARAUJO LIMA, G. M
Format: Article
Language:English
Subjects:
Animals
Antibodies, Protozoan - immunology
Antigens, Protozoan - immunology
Biological and medical sciences
Experimental protozoal diseases and models
Hypersensitivity, Delayed - immunology
Immunization
Infectious diseases
Leishmania mexicana - immunology
Leishmania mexicana - pathogenicity
Leishmaniasis, Cutaneous - immunology
Leishmaniasis, Cutaneous - prevention & control
Lymphocyte Activation
Medical sciences
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Parasitic diseases
Protozoal diseases
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Summary:The induction of protective immunity to Leishmania amazonensis was investigated by injection of parasite clones of low and medium virulence into susceptible mice. To this end, L. amazonensis were cloned by limiting dilution and the clones' virulence was evaluated by the course of infection in susceptible mice. Clones originally derived from the spleen showed virulence variations in comparison with that of the parental population (PP) of parasites. Two low-virulence clones (SP 5 and SP 20) and one medium-virulence clone (SP 11), representative of the spectrum of derived clones, were compared with virulent parasites and with an avirulent strain (Josefa) as to their ability to induce T-cell immune responses and to protect BALB/c mice from infection with the virulent L. amazonensis PP. Clone SP 20 and clone SP 11 induced partial protection when injected by the intravenous and intradermal route, respectively. The avirulent Josefa strain induced neither T-cell responses nor protection. Low-virulence L. amazonensis clones can therefore be additional tools in vaccine investigation.
ISSN:0932-0113
1432-1955
DOI:10.1007/s004360050321