Formate and ethanol are the major products of glycerol fermentation produced by a Klebsiella planticola strain isolated from red deer

The rumen contents of red deer (Cervus elaphus) were used to isolate bacteria capable of fermenting glycerol. The biochemistry, physiology, morphology and phylogeny of one isolate were studied in detail. The isolate (DR3) was tentatively identified as a strain of the species Klebsiella planticola as...

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Bibliographic Details
Published in:Journal of applied microbiology 1997-08, Vol.83 (2), p.166-174
Main Authors: Jarvis, G.N., Moore, E.R.B., Thiele, J.H.
Format: Article
Language:English
Subjects:
Animals
Bacteriology
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Central Nervous System Depressants - metabolism
Deer - microbiology
Ethanol - metabolism
Fermentation - physiology
Formates - metabolism
Fundamental and applied biological sciences. Psychology
Genes, Bacterial - genetics
Glycerol - metabolism
Hemostatics - metabolism
Isolation and description
Klebsiella - genetics
Klebsiella - metabolism
Klebsiella - ultrastructure
Klebsiella planticola
Metabolism. Enzymes
Microbiology
Microscopy, Electron
Mission oriented research
Molecular Sequence Data
Phenotype
Phylogeny
RNA, Ribosomal, 16S - isolation & purification
Rumen - microbiology
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Summary:The rumen contents of red deer (Cervus elaphus) were used to isolate bacteria capable of fermenting glycerol. The biochemistry, physiology, morphology and phylogeny of one isolate were studied in detail. The isolate (DR3) was tentatively identified as a strain of the species Klebsiella planticola as based on phenotypic characterization. The data obtained from 16S rRNA sequence analysis showed that the deer rumen isolate DR3 was 99·7% similar to the type strain of Kl. planticola (DSM 3069T), thus confirming the results of the phenotypic characterization. During active cell growth, it was established that glycerol dissimilation by Kl. planticola DR3 led to the production of formate and ethanol at equimolar levels of 32 mmol l−1 and 30 mmol l−1, respectively. As a result of the data obtained, a closed carbon balance was constructed for Kl. planticola DR3. This finding represented the first report of the complete end‐product profile for glycerol dissimilation by a strain of Kl. planticola isolated from cervine rumen contents.
ISSN:1364-5072
1365-2672
DOI:10.1046/j.1365-2672.1997.00217.x