Cloning and characterization of subtracted cDNAs from a human ciliary body library encoding TIGR, a protein involved in juvenile open angle glaucoma with homology to myosin and olfactomedin

A group of cDNAs isolated from a subtractive ciliary body library of a normal human eye donor revealed 100% identity with TIGR a candidate gene responsible for juvenile open angle glaucoma [Science 275 (1997) 668–670]. Several structural features of the deduced human protein have been noted: a cleav...

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Bibliographic Details
Published in:FEBS letters 1997-08, Vol.413 (2), p.349-353
Main Authors: Ortego, Javier, Escribano, Julio, Coca-Prados, Miguel
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
Ciliary Body - chemistry
Ciliary Body - physiology
Cloning, Molecular
Cytoskeletal Proteins
DNA, Complementary - genetics
Extracellular Matrix Proteins - genetics
Eye - chemistry
Eye Proteins - genetics
Gene Library
Glaucoma, Open-Angle - genetics
Glycoproteins - genetics
Human ocular ciliary body
Humans
Molecular Sequence Data
mRNA expression
Myosin
Myosins - genetics
Olfactomedin
Organ Specificity
RNA, Messenger - analysis
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Subtracted cDNAs
TIGR gene
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Summary:A group of cDNAs isolated from a subtractive ciliary body library of a normal human eye donor revealed 100% identity with TIGR a candidate gene responsible for juvenile open angle glaucoma [Science 275 (1997) 668–670]. Several structural features of the deduced human protein have been noted: a cleavable N-terminal signal peptide, a periodic repetition at the N-terminus of leucine and arginine residues at every seventh and eleven position respectively in helix conformation (leucine zipper-like motif) exhibiting homology with myosin, and with olfactomedin in the C-terminus. The mRNA for TIGR is abundantly expressed in the ciliary body, iris, heart and skeletal muscle.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(97)00934-4