Atomic force microscopy of plant cell walls, plant cell wall polysaccharides and gels

Methods developed for the routine imaging of polysaccharides by atomic force microscopy (AFM) have been used to image plant polysaccharides from higher plants (pectin) and algae (carrageenan). These methods have been extended to image K-carrageenan association in hydrated films. Finally, AFM has bee...

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Bibliographic Details
Published in:International journal of biological macromolecules 1997-08, Vol.21 (1), p.61-66
Main Authors: Morris, V.J, Gunning, A.P, Kirby, A.R, Round, A, Waldron, K, Ng, A
Format: Article
Language:English
Subjects:
AFM
Carrageenan
Cell Wall - ultrastructure
Cell walls
Gelation
Gels
Microscopy, Atomic Force - methods
Pectin
Plants - ultrastructure
Polysaccharides - chemistry
Space life sciences
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Summary:Methods developed for the routine imaging of polysaccharides by atomic force microscopy (AFM) have been used to image plant polysaccharides from higher plants (pectin) and algae (carrageenan). These methods have been extended to image K-carrageenan association in hydrated films. Finally, AFM has been used to image polysaccharide architecture in moist plant cell walls. Simple experimental and image processing methods have been used to enhance molecular structure in `rough' cell wall surfaces.
ISSN:0141-8130
1879-0003
DOI:10.1016/S0141-8130(97)00042-1