Determination of naphthodianthrones in plant extracts from Hypericum perforatum L. by liquid chromatography–electrospray mass spectrometry

The determination of the naphthodianthrone constituents in extracts of dried blossoms of Hypericum perforatum L. by combined HPLC–electrospray mass spectrometry is described. Hypericin (1), pseudohypericin (2) and their precursor compounds produce intensive negative quasi-molecular ions by deprotona...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1997-08, Vol.695 (2), p.309-316
Main Authors: Piperopoulos, Georgios, Lotz, Ralf, Wixforth, Annette, Schmierer, Thilo, Zeller, Klaus-Peter
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chromatography, High Pressure Liquid
General pharmacology
Hypericin
Mass Spectrometry
Medical sciences
Naphthodianthrones
Perylene - analogs & derivatives
Perylene - chemistry
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - chemistry
Plants, Medicinal - chemistry
Protohypericin
Protopseudohypericin
Pseudohypericin
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Summary:The determination of the naphthodianthrone constituents in extracts of dried blossoms of Hypericum perforatum L. by combined HPLC–electrospray mass spectrometry is described. Hypericin (1), pseudohypericin (2) and their precursor compounds produce intensive negative quasi-molecular ions by deprotonation provided a non-acidic eluent system is used in the HPLC separation. From the [M–H] − ions formed in the electrospray ionization process characteristic daughter ion spectra can be obtained by collisional activation which have been studied by tandem mass spectrometry.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(97)00188-6