A New Flavone C-Glycoside and Antiplatelet and Vasorelaxing Flavones from Gentiana arisanensis

• Published in: Journal of natural products (Washington, D.C.) D.C.), 1997-08, Vol.60 (8), p.851-853
• Main Authors: Lin, Chun-Nan, Kuo, Shih-Hsien, Chung, Mei-Ing, Ko, Feng-Nien, Teng, Che-Ming
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; BLOOD COAGULATION FACTORS; BLOOD PROTEINS; CHEMICAL COMPOSITION; CHEMICAL STRUCTURE; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; DRUG PLANTS; ESPECTROMETRIA; ESTRUCTURA QUIMICA; FLAVONOIDE; FLAVONOIDES; FLAVONOIDS; Flavonoids - chemistry; Flavonoids - isolation & purification; Flavonoids - pharmacology; General pharmacology; GENTIANA; GLICOSIDOS; GLYCOSIDE; GLYCOSIDES; In Vitro Techniques; ISOVITEXIN; ISOVITEXIN 6'-O-GLUCOSIDE; LUTEOLIN-7-O-BETA-GLUCOSIDE; Male; Medical sciences; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; PLANTAS MEDICINALES; PLANTE MEDICINALE; Plants - chemistry; Platelet Aggregation Inhibitors - chemistry; Platelet Aggregation Inhibitors - isolation & purification; Platelet Aggregation Inhibitors - pharmacology; PROTEINAS SANGUINEAS; PROTEINE SANGUINE; QUERCETIN; QUERCETINA; QUERCETINE; Rabbits; Rats; Rats, Wistar; SPECTRAL ANALYSIS; SPECTROMETRIE; SPECTROMETRY; Spectrum Analysis; STRUCTURE CHIMIQUE; TAIWAN; Vasodilator Agents - chemistry; Vasodilator Agents - isolation & purification; Vasodilator Agents - pharmacology
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/np970011e

A new flavone C-glycoside, isovitexin 6‘‘-O-glucoside (1), and three known flavonoids, quercetin, isovitexin, and luteolin-7-O-β-d-glucoside, have been further isolated from the whole plant of Gentiana arisanensis Hayata. The new compound was characterized by spectral methods and chemical reactions. The antiplatelet effects of isovitexin 6‘‘-O-glucoside (1), isoorientin (2), 2 peracetate (3), isovitexin (4), luteolin 7-O-β-d-glucoside (5), luteolin (6), isoorientin 6‘‘-O-glucoside (7), and 7 peracetate (8) were studied using washed rabbit platelets. Of the compounds tested, 6 showed potent antiplatelet effects on arachidonic acid (AA)-induced platelet aggregation (IC50 = 43.5 μM). The effect of 2, 5, and 6 on the contraction of rat thoracic aorta was also studied. Compound 6 depressed markedly the contraction induced by Ca2+ (1.9 mM) in high-K+ (80 mM) medium, with an IC50 of about 156 μM and also inhibited noradrenaline (3 μM)-induced phasic and tonic contractions, with an IC50 of about 68 and 72 μM, respectively.