Interleukin-6 and Interleukin-1 Production in Acute Leukemia With Monocytoid Differentiation

• Published in: Blood 1989-11, Vol.74 (6), p.2081-2087
• Main Authors: van der Schoot, C. Ellen, Jansen, Patty, Poorter, Mirella, Wester, Mieke R., von dem Borne, Albert E.G. Kr, Aarden, Lucien A., van Oers, Rien H.J.
• Format: Article
• Language: English
• Subjects: Antigens, CD34; Antigens, Differentiation; Antigens, Differentiation, Myelomonocytic; Biological and medical sciences; Blotting, Northern; Cell Adhesion; Cell Differentiation; Hematologic and hematopoietic diseases; Humans; In Vitro Techniques; Interleukin-1 - biosynthesis; Interleukin-6 - biosynthesis; Interleukin-6 - genetics; Leukemia, Monocytic, Acute - metabolism; Leukemia, Myelomonocytic, Acute - metabolism; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Lipopolysaccharide Receptors; Medical sciences; RNA, Messenger - genetics
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V74.6.2081.2081

Several authors have reported the in vitro production of colony-stimulating factors (CSF) and interleukin-1 (IL-1) by the neoplastic cells from patients with acute myeloid leukemia (AML). Using a sensitive bioassay for IL-6, the capacity of the leukemic cells of 30 patients with AML to produce IL-6 was examined. IL-6 production was found to be specific for cells from patients with an AML with monocytic differentiation (12 of 15 M4 and M5 patients, 0 of 15 M1 and M2 patients). Moreover, IL-6 production was paralleled by IL-1 production. The IL-6- and IL-1–producing cells were mainly found in the more mature monocytic cell fractions, defined as CD14-positive and CD34-negative adherent cells. By limiting dilution experiments, it could be excluded that the production of IL-1 or IL-6 was due to contamination with normal monocytes.