Primed in situ (PRINS) Labelling with Alu and Satellite Primers for Rapid Characterization of Human Chromosomes in Hybrid Cell Lines

The primed in situ (PRINS) labelling method was developed as an alternative to classical cytogenetics and fluorescence in situ hybridization (FISH) for the characterization of interspecific somatic hybrids. Full karyotypes were performed by PRINS using Alu-specific primers to generate the painting o...

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Bibliographic Details
Published in:Chromosome research 1997-08, Vol.5 (5), p.307-312
Main Authors: Coullin, P, Andréo, B, Charlieu, J P, Candelier, J J, Pellestor, F
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Centromere - genetics
CHO Cells
Chromosome Banding
Chromosomes
Chromosomes, Human - genetics
Cricetinae
DNA Primers - genetics
Fibroblasts
Humans
In Situ Hybridization - methods
Interphase - genetics
Karyotyping
Mice
Microsatellite Repeats
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Summary:The primed in situ (PRINS) labelling method was developed as an alternative to classical cytogenetics and fluorescence in situ hybridization (FISH) for the characterization of interspecific somatic hybrids. Full karyotypes were performed by PRINS using Alu-specific primers to generate the painting of all human material associated with R-like banding. The representativity of individual human chromosomes was established using primers specific for discriminent α-satellite DNA sequences providing specific signals on the centromeres of the targeted chromosomes and corresponding spots in interphase nuclei. Using this methodology, a somatic hybrid clone was shown to be monochromosomal for the der(11) from a t(11;22) patient.
ISSN:0967-3849
1573-6849
DOI:10.1023/b:chro.0000038761.15856.4d