Cloning and sequencing of the Clostridium perfringens enterotoxin gene

Several gene banks of Clostridium perfringens in E. coli were constructed. Using a mixture of synthetic 29-mer DNA probes clones were selected containing inserts from the C. perfringens gene coding for the enterotoxin. This has allowed sequencing of the complete gene and its flanking regions. The de...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 1989-08, Vol.56 (2), p.181-190
Main Authors: VAN DAMME-JONGSTEN, M, WERNARS, K, NOTERMANS, S
Format: Article
Language:English
Subjects:
Bacteriology
Base Sequence
Biological and medical sciences
Cloning, Molecular
DNA Probes
Enterotoxins - genetics
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Microbiology
Molecular Sequence Data
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
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Summary:Several gene banks of Clostridium perfringens in E. coli were constructed. Using a mixture of synthetic 29-mer DNA probes clones were selected containing inserts from the C. perfringens gene coding for the enterotoxin. This has allowed sequencing of the complete gene and its flanking regions. The decuded amino acid sequence (320 a.a.) was found to differ at several sites from the sequence published previously by others. Two 40-mer DNA-probes were used to detect the toxin gene in C. perfringens strains isolated from the faeces of different non-symptomatic animals. Only 6% of the strains were found to possess the gene.
ISSN:0003-6072
1572-9699
DOI:10.1007/bf00399981