Mapping of peptides and protein fragments in human urine using liquid chromatography–mass spectrometry

A method for the mapping of peptide mixtures, heterogeneous with respect to the concentration and the size of individual peptides, was established with the aim of obtaining a comprehensive analysis of human urine peptides. Peptide extraction and fractionation were optimized to achieve a two-step ana...

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Bibliographic Details
Published in:Journal of Chromatography A 1997-07, Vol.776 (1), p.117-124
Main Authors: Heine, Gabriele, Raida, Manfred, Forssmann, Wolf-Georg
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Aminoacids, peptides. Hormones. Neuropeptides
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Female
Fundamental and applied biological sciences. Psychology
Humans
Male
Mass Spectrometry
Molecular Sequence Data
Peptide Fragments - urine
Peptide Mapping - methods
Proteins
Sequence Analysis
Spectrophotometry, Ultraviolet
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Description
Summary:A method for the mapping of peptide mixtures, heterogeneous with respect to the concentration and the size of individual peptides, was established with the aim of obtaining a comprehensive analysis of human urine peptides. Peptide extraction and fractionation were optimized to achieve a two-step analysis, using reversed-phase and ion-exchange chromatography. Highly sensitive detection of peptides was performed by coupling microbore HPLC with electrospray mass spectrometry (ESI-MS). Peptides such as urodilatin, angiotensin and fragments of psoriasin, granulin and uromodulin were isolated and sequenced. The procedure presented here is a tool for the analysis of complex peptide mixtures from human urine.
ISSN:0021-9673
DOI:10.1016/S0021-9673(97)00440-8