Cryptosporidiosis-Induced Impairment of Ion Transport and Na/-Glucose Absorption in Adult Immunocompromised Mice

• Published in: The Journal of infectious diseases 1997-09, Vol.176 (3), p.834-837
• Main Authors: Kapel, Nathalie, Huneau, Jean Francois, Magne, Denis, Tomé, Daniel, Gobert, Jean-Gérard
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Concise Communications; Cryptosporidiosis; Cryptosporidiosis - metabolism; Cryptosporidium parvum; Diarrhea; Disease Models, Animal; Epithelial cells; Experimental protozoal diseases and models; Glucose - metabolism; Ileum; Ileum - metabolism; Immunocompromised Host; Immunocompromised populations; Infections; Infectious diseases; Intestinal Absorption; Ion Transport; Medical sciences; Mice; Mice, SCID; Oocysts; Parasitic diseases; Piglets; Protozoal diseases; Sodium - metabolism
• ISSN: 0022-1899; 1537-6613
• DOI: 10.1086/517316

Electrolyte transport was investigated during chronic cryptosporidiosis in adult anti-interferon-γ treated SCID mice by means of Ussing chamber techniques. In basal conditions, infection of immunocompromised mice with Cryptosporidium parvum resulted in a 30% reduction (P < .05) in the ileal short-circuit (Isc) current related to a 28% reduction (P < .05) in tissue conductance compared with controls. The rises in Isc and transepithelial potential difference induced by glucose (10 mM) were significantly reduced by Cryptosporidium infection (P < .01) compared with controls. In contrast, responses to mucosal glutamine were marginally affected. Electrical parameters of the ileum were not affected by the addition of indomethacin or furosemide, in either control or Cryptosporidium-infected mice. Thus, long-term cryptosporidiosis in immunocompromised animals leads to a reduction in net ion exchanges, decreased paracellular shunting, and impaired Na+- glucose cotransport in the ileum, without prostanoid- or enterotoxin-mediated electrogenic Cl− secretion.