Histamine release from human bronchoalveolar lavage mast cells by neurokinin A and bradykinin

This study examined whether bradykinin and neurokinin A activate human pulmonary mast cells retrieved by bronchoalveolar lavage (BAL). BAL samples were obtained at routine bronchoscopy from 14 unpreselected patients. Histamine release experiments were performed using substance P, neurokinin A, brady...

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Bibliographic Details
Published in:Inflammation research 1997-08, Vol.46 (8), p.306-309
Main Authors: Cross, L J, Heaney, L G, Ennis, M
Format: Article
Language:English
Subjects:
Bradykinin - pharmacology
Bronchoalveolar Lavage Fluid - cytology
Calcimycin - pharmacology
Female
Histamine Release
Humans
Ionophores - pharmacology
Male
Mast Cells - drug effects
Mast Cells - physiology
Middle Aged
Neurokinin A - pharmacology
p-Methoxy-N-methylphenethylamine - pharmacology
Substance P - pharmacology
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Summary:This study examined whether bradykinin and neurokinin A activate human pulmonary mast cells retrieved by bronchoalveolar lavage (BAL). BAL samples were obtained at routine bronchoscopy from 14 unpreselected patients. Histamine release experiments were performed using substance P, neurokinin A, bradykinin (peptides 25 and 50 microM), compound 48/80 (0.75-10 micrograms/ml) and A23187 (1 microM). Statistical analyses were performed using the paired Student's t-test and Pearson's linear correlation coefficient. Compound 48/80 induced release was significantly lower than that induced by the other secretagogues (p < 0.05). Neurokinin A and bradykinin induced release correlated significantly with substance P induced release (p < 0.01), suggesting similar mechanisms of action. No correlations were observed between neurokinin A or bradykinin-induced release and the non-peptide stimuli studied. The mechanism of neurokinin A- and bradykinin-induced bronchoconstriction is not yet clear but our data suggest an indirect effect mediated by mast cell degranulation.
ISSN:1023-3830
1420-908X
DOI:10.1007/s000110050192