Lipid lateral diffusion and membrane organization

It is shown that investigating the lateral motion of lipids in biological membranes can provide useful information on membrane lateral organization. After labeling membranes with extrinsic or intrinsic lipophilic fluorescent probes, fluorescence recovery after photobleaching experiments strongly sug...

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Bibliographic Details
Published in:FEBS Letters 1989-10, Vol.257 (1), p.10-16
Main Authors: Tocanne, Jean-François, Dupou-Cézanne, Laurence, Lopez, André, Tournier, Jean-François
Format: Article
Language:English
Subjects:
acyl-ANnoGroPCho, sn-1-acyl-sn-2-[9-(2-anthryl)-nonanoyl]-glycero-3-phosphocholine
Animals
ANno, 9-(2-anthryl)-nonanoic acid
ASte, 12-(9-anthroyloxy)-stearic acid
Biological and medical sciences
C14-, C16-, C18-diI/diO, 1,1'-ditetradecyl-, 1,1'-dihexadecyl-, 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine/oxatricarbocyanine
C18-Rh, octadecylrhodamine B
C4-Fl, C18-Fl, 5-(N-tetradecyl)-, 5-(N-octadecyl)-aminofluorescein
Cell membranes. Ionic channels. Membrane pores
Cell structures and functions
Diffusion
Fl-PE, N-fluorescein-N'-phosphatidylethanolamine
for the sake of clarity, lipid probes are abbreviated as they are in the reference papers. C12NFlu, 5-(N-dodecanoyl)-aminofluorescein
Fundamental and applied biological sciences. Psychology
HEDAF or C16-Fl or Pam-aminofluorescein, 5-(N-hexadecanoyl)-aminofluorescein
Membrane Lipids - physiology
Membranes - physiology
Membranes - ultrastructure
Molecular and cellular biology
NBC-PE, N-4-nitrobenzo-2-oxa-1,3-diazol-4-ylphosphatidylethanolamine
NBD-PC, 1-acyl-2-(N-4-nitrobenzo-2-oxa-1,3-diazol)-aminocaproyl- (or aminohexanoyl, or aminododecanoyl)-phosphatidylcholine
NBD-PtdCho, -PtdEtn, -PtdSer, 1-acyl-2-[12-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)]-aminododecanoyl-phosphatidylcholine, -phosphatidylethanolamine, phosphatidylserine
reviews
Rho-PE, rhodamine-phosphatidylethanolamine
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Summary:It is shown that investigating the lateral motion of lipids in biological membranes can provide useful information on membrane lateral organization. After labeling membranes with extrinsic or intrinsic lipophilic fluorescent probes, fluorescence recovery after photobleaching experiments strongly suggests that specialized cells like spermatozoa, eggs and epithelia exhibit surface membrane regionalization or macrocompartmentation and that lateral microheterogeneities or lipid microdomains exist in the plasma membrane of many cellular systems. Phospholipid; Photobleaching; Lateral diffusion; Membrane organization; Macrodomain; Microdomain
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(89)81774-0