Distribution of the total unsaturation in lipid components of plasma as a new differential diagnostic method in clinical analysis

Using ozonization and thin-layer chromatographic methods we determined the qualitative and quantitative correlation of unsaturation distribution (UD) in individual fractions of blood plasma lipids in children suffering from insulin-dependent diabetes mellitus (IDDM). The research was aimed at elucid...

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Bibliographic Details
Published in:Journal of Chromatography A 1997-08, Vol.777 (1), p.47-50
Main Authors: Poznyak, T.I, Kiseleva, E.V, Turkina, T.I
Format: Article
Language:English
Subjects:
Adolescent
Chemistry, Clinical - methods
Child
Child, Preschool
Chromatography, Thin Layer - methods
Cohort Studies
Diabetes Mellitus, Type 1 - blood
Diabetes Mellitus, Type 1 - diagnosis
Diabetes Mellitus, Type 1 - metabolism
Diabetic Ketoacidosis - blood
Diabetic Ketoacidosis - diagnosis
Diagnosis, Differential
Humans
Infant
Lipids
Lipids - blood
Lipids - chemistry
Ozone - metabolism
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Summary:Using ozonization and thin-layer chromatographic methods we determined the qualitative and quantitative correlation of unsaturation distribution (UD) in individual fractions of blood plasma lipids in children suffering from insulin-dependent diabetes mellitus (IDDM). The research was aimed at elucidation of biochemical criterion of the degree of metabolic disorders in children with IDDM and at development of methods for quantitative assessment of such disorders. Twenty children were examined during the compensation stage (group 1), and twelve during decompensation with ketoacidosis (group 2). The present investigation shows that in the case of insulin-dependent diabetes mellitus in children the total unsaturation distribution (TUD) in plasma lipid fractions were found to be decreased significantly compared to healthy controls. The pattern of TUD in plasma lipid fractions may serve as a new biochemical criterion for metabolic disorders and decompensation in IDDM.
ISSN:0021-9673
DOI:10.1016/S0021-9673(97)00559-1