Rapid Analysis of Hemoglobin from Whole Human Blood by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry

Matrix‐assisted laser desorption time‐of‐flight mass spectrometry (MALDI‐TOFMS) is applied to the analysis of whole human blood. No separation or purification steps are required to observe individual globins and the sensitivity of the method is excellent. Mass accuracy and precision are 0.01% using...

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Bibliographic Details
Published in:Rapid communications in mass spectrometry 1997-01, Vol.11 (13), p.1435-1439
Main Authors: Houston, Christopher T., Reilly, James P.
Format: Article
Language:English
Subjects:
Hemoglobin A - analysis
Hemoglobin, Sickle - analysis
Hemoglobins - analysis
Heterozygote
Humans
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Online Access:Get full text
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Summary:Matrix‐assisted laser desorption time‐of‐flight mass spectrometry (MALDI‐TOFMS) is applied to the analysis of whole human blood. No separation or purification steps are required to observe individual globins and the sensitivity of the method is excellent. Mass accuracy and precision are 0.01% using a linear TOF system with pulsed ion extraction. Sickle‐cell hemoglobin is easily distinguishable from normal hemoglobin. The speed and simplicity of the method make it potentially attractive for large‐scale population screening. © 1997 John Wiley & Sons, Ltd.
ISSN:0951-4198
1097-0231
DOI:10.1002/(SICI)1097-0231(19970830)11:13<1435::AID-RCM24>3.0.CO;2-A