Reactivity of monoclonal antibody P1 with glomerular basement membrane in thin-membrane nephropathy

A monoclonal antibody, P1, possessing antiglomerular basement membrane specificity similar to that of naturally-occurring Goodpasture antibody, was used to study renal biopsy material from patients with thin-membrane nephropathy. Immunofluorescence and immunoperoxidase techniques were employed to de...

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Bibliographic Details
Published in:Nephrology, dialysis, transplantation dialysis, transplantation, 1989, Vol.4 (7), p.611-617
Main Authors: DISCHE, F. E, BROOKE, I. P, CASHMAN, S. J, SEVERN, A, TAUBE, D, PARSONS, V, KERSHAW, M, REED, A, PUSEY, C. D
Format: Article
Language:English
Subjects:
Adult
Aged
Antibodies, Monoclonal
Basement Membrane - immunology
Biological and medical sciences
Diagnosis, Differential
Female
Glomerulonephritis
Glomerulonephritis, Membranous - diagnosis
Glomerulonephritis, Membranous - immunology
Humans
Immunohistochemistry
Kidney Glomerulus - immunology
Male
Medical sciences
Middle Aged
Nephritis, Hereditary - diagnosis
Nephritis, Hereditary - immunology
Nephrology. Urinary tract diseases
Nephropathies. Renovascular diseases. Renal failure
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Summary:A monoclonal antibody, P1, possessing antiglomerular basement membrane specificity similar to that of naturally-occurring Goodpasture antibody, was used to study renal biopsy material from patients with thin-membrane nephropathy. Immunofluorescence and immunoperoxidase techniques were employed to detect tissue localisation after sections had been incubated with P1. Staining of glomerular and tubular basement membranes in the 14 patients with thin-membrane nephropathy was similar to that in 10 subjects with various other renal diseases, whereas three patients with Alport's syndrome all gave diminished or absent staining, as has been reported previously. These observations confirm that Goodpasture antigen is present in the basement membranes in thin-membrane nephropathy and that it reacts normally with P1 antibody. They also add to the evidence that the lesions of thin-membrane nephropathy and Alport's syndrome are fundamentally different. The staining method may be used as a differential diagnostic test.
ISSN:0931-0509
1460-2385
1460-2385
DOI:10.1093/ndt/4.7.611