Lipids of hamster cheek pouch epithelium

The hamster cheek pouch is a much used but incompletely understood experimental model. In particular, the cheek pouch epithelial lipids, which are important for permeability barrier function as well as other aspects of epithelial biology, have not been completely characterized. In the present study,...

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Bibliographic Details
Published in:Lipids 1997-09, Vol.32 (9), p.961-964
Main Authors: Whittle, Sara, Swartzendruber, Donald C., Kremer, Mary, Squier, Christopher A., Wertz, Philip W.
Format: Article
Language:English
Subjects:
Animals
Ceramides - analysis
Ceramides - chemistry
Cheek
Chromatography, Thin Layer
Cricetinae
Epidermis - chemistry
Epithelium - chemistry
Epithelium - ultrastructure
Esters
Lipids
Lipids - analysis
Lipids - chemistry
Mesocricetus
Phospholipids - analysis
Proteins
Sterols - analysis
Thin layer chromatography
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Summary:The hamster cheek pouch is a much used but incompletely understood experimental model. In particular, the cheek pouch epithelial lipids, which are important for permeability barrier function as well as other aspects of epithelial biology, have not been completely characterized. In the present study, the complete lipid class composition has been determined by thin‐layer chromatography in conjunction with photodensitometry. The major lipid classes were phospholipids, free sterols, and ceramides. Minor amounts of monohexosylceramides, sterol esters, fatty acids, and triglycerides were also present. Significant amounts of covalently bound ω‐hydroxyceramide was also detected. Transmission electron micrographs reveal extensive, largely paired, lipid bilayers in the intercellular spaces of the stratum corneum.
ISSN:0024-4201
1558-9307
DOI:10.1007/s11745-997-0124-y