Transmembrane Domain of the Envelope Gene of a Polycythemia-Inducing Retrovirus Determines Erythropoietin-Independent Growth

Both the polycythemia-inducing and the anemia-inducing strains of Friend spleen focus-forming virus can induce acute erythroleukemia in susceptible adult mice. However, only cells infected with the polycythemia-inducing strain become erythropoietin-independent for proliferation and differentiation....

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-10, Vol.86 (20), p.7957-7960
Main Authors: Chung, Siu-Wah, Wolff, Linda, Ruscetti, Sandra K.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Cell Differentiation - drug effects
Cell Division - drug effects
Cells, Cultured
Colony-Forming Units Assay
DNA Replication
env genes
Epics
Erythroid cells
Erythropoietin - pharmacology
Genes, Viral
Glycoproteins
Leukemia Virus, Murine - genetics
Liver cells
Membrane proteins
Mice
Mice, Inbred Strains
Molecular Sequence Data
Plasmids
Polycythemia - microbiology
Polycythemia - pathology
Spleen - microbiology
Spleen - pathology
Spleen cells
Spleen focus forming viruses
Spleen Focus-Forming Viruses - genetics
Spleen Focus-Forming Viruses - pathogenicity
Viral Envelope Proteins - genetics
Viral Structural Proteins - genetics
Viruses
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Summary:Both the polycythemia-inducing and the anemia-inducing strains of Friend spleen focus-forming virus can induce acute erythroleukemia in susceptible adult mice. However, only cells infected with the polycythemia-inducing strain become erythropoietin-independent for proliferation and differentiation. The sequences responsible for the altered erythropoietin responsiveness have previously been localized to a 678-base-pair EcoRI-Cla I fragment at the 3′end of the envelope gene. This region is now further analyzed by dividing it into two fragments by using the Fok I restriction site. Two recombinants were made by replacing either the 558-base-pair EcoRI-Fok I or the 113-base-pair Fok I-Cla I env gene fragments from the anemia-inducing strain of spleen focus-forming virus with sequences derived from the polycythemia-inducing strain. Our results indicate that the 113-base-pair Fok I-Cla I fragment, which encodes primarily the transmembrane domain of the envelope protein, determines erythropoietin-independent growth.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.20.7957