Garlic Compounds Protect Vascular Endothelial Cells from Oxidized Low Density Lipoprotein-induced Injury

Oxidation of low density lipoprotein (LDL) has been recognized as playing an important role in the initiation and progression of atherosclerosis. In this study, the effects of aged garlic extract and one of its major compounds, S‐allylcysteine, on oxidized LDL‐induced cell injury were studied. Pulmo...

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Bibliographic Details
Published in:Journal of pharmacy and pharmacology 1997-09, Vol.49 (9), p.908-911
Main Authors: IDE, NAGATOSHI, LAU, BENJAMIN H. S.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
Cell Line
Cell Survival - drug effects
Cysteine - analogs & derivatives
Cysteine - pharmacology
Endothelium, Vascular - cytology
Endothelium, Vascular - drug effects
Endothelium, Vascular - enzymology
Endothelium, Vascular - pathology
Garlic
General pharmacology
In Vitro Techniques
L-Lactate Dehydrogenase - metabolism
Lipid Peroxidation - drug effects
Lipoproteins, LDL - pharmacology
Medical sciences
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - pharmacology
Plants, Medicinal
Pulmonary Artery - cytology
Pulmonary Artery - pathology
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Summary:Oxidation of low density lipoprotein (LDL) has been recognized as playing an important role in the initiation and progression of atherosclerosis. In this study, the effects of aged garlic extract and one of its major compounds, S‐allylcysteine, on oxidized LDL‐induced cell injury were studied. Pulmonary artery endothelial cells were pre‐incubated with the garlic extract (1, 2.5 and 5 mg mL−) or 5‐allylcysteine (01, 1, 10 and 20 mm) at 37°C and 5% CO2 for 24 h, washed, and then exposed to 01 mg mL− oxidized LDL for 24 h. Lactate dehydrogenase release as an index of membrane damage, methylthiazol tetrazolium assay for cell viability and thiobarbituric acid reactive substances indicating lipid peroxidation were determined. Preincubation of endothelial cells with the extract or S‐allylcysteine significantly prevented membrane damage, loss of cell viability and lipid peroxidation. The data indicate that these compounds can protect vascular endothelial cells from injury caused by oxidized LDL, and suggest that they may be useful for prevention of atherosclerosis.
ISSN:0022-3573
2042-7158
DOI:10.1111/j.2042-7158.1997.tb06134.x