Thymotaxin, a chemotactic protein, is identical to beta 2-microglobulin

• Published in: Science (American Association for the Advancement of Science) 1989-11, Vol.246 (4931), p.803-806
• Main Authors: Dargemont, C, Dunon, D, Deugnier, M A, Denoyelle, M, Girault, J M, Lederer, F, Lê, K H, Godeau, F, Thiery, J P, Imhof, B A
• Format: Article
• Language: English
• Subjects: Animals; beta 2-Microglobulin - pharmacology; Biology; Bone Marrow Cells; Cell Line; Cell Movement - drug effects; Chemistry; Chemotactic Factors - pharmacology; Chemotaxis; Chromatography, Gel; Chromatography, High Pressure Liquid; Electrophoresis, Polyacrylamide Gel; Granulocytes - drug effects; Hematopoietic Stem Cells - drug effects; Rats
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.2683083

Thymotaxin, an 11-kilodalton protein chemotactic for rat bone marrow hematopoietic precursors, was purified from media conditioned by a rat thymic epithelial cell line. The NH2-terminal sequence of thymotaxin was identical to that of rat beta 2-microglobulin (beta 2m). Antibodies to beta 2m removed thymotaxin activity from the fraction containing the 11-kilodalton protein. Chemotactic activity was observed with rat plasma beta 2m, human beta 2m, and mouse recombinant beta 2m, further supporting the identity of thymotaxin with beta 2m. The directional migration, as opposed to random movement, of the cells was also confirmed. The only rat bone marrow cells that migrated toward beta 2m were Thy1+ immature lymphoid cells devoid of T cell, B cell, and myeloid cell differentiation markers.