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v-erbA oncogene initiates ultrastructural changes characteristic of early and intermediate events of meiotic maturation in Xenopus oocytes

The growth‐promoting properties of the retroviral v‐erbA oncogene, a highly mutated version of the chicken thyroid hormone receptor (TR) α, have so far exclusively been linked to dominant repression of the antimitogenic roles of TR and retinoic acid receptors. Here we show that when expressed in Xen...

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Published in:Journal of cellular biochemistry 1997-11, Vol.67 (2), p.184-200
Main Authors: Nagl, Sylvia B., Bunn, Caroline F., Allison, Lizabeth A.
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Language:English
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Bunn, Caroline F.
Allison, Lizabeth A.
description The growth‐promoting properties of the retroviral v‐erbA oncogene, a highly mutated version of the chicken thyroid hormone receptor (TR) α, have so far exclusively been linked to dominant repression of the antimitogenic roles of TR and retinoic acid receptors. Here we show that when expressed in Xenopus oocytes v‐ErbA induced ultrastructural changes characteristic of early and intermediate events of meiotic maturation by activating gene transcription. v‐ErbA–induced maturation events occurred without activation of the cAMP/maturation‐promoting factor signal pathway and were arrested prior to meiotic spindle formation. The effects of v‐ErbA were not mimicked by a dominant negative in vitro–generated mutant of human TR, suggesting that v‐ErbA can contribute to cell cycle reentry by interference with regulatory pathways distinct from those involving TR. Interestingly, a portion of v‐ErbA expressed in oocytes was present at the cytoplasmic fibrils of the nuclear pore complexes, suggesting that in addition to its intranuclear function v‐ErbA may modulate nucleocytoplasmic transport. J. Cell. Biochem. 67:184–200, 1997. © 1997 Wiley‐Liss, Inc.
doi_str_mv 10.1002/(SICI)1097-4644(19971101)67:2<184::AID-JCB4>3.0.CO;2-T
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Biochem. 67:184–200, 1997. © 1997 Wiley‐Liss, Inc.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/(SICI)1097-4644(19971101)67:2&lt;184::AID-JCB4&gt;3.0.CO;2-T</identifier><identifier>PMID: 9328824</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological Transport ; Cell Nucleus - metabolism ; Cell Nucleus - ultrastructure ; Cyclic AMP - physiology ; Cytoplasm - metabolism ; Female ; Gene Expression ; Gene Transfer Techniques ; Genes, erbA ; Humans ; maturation-promoting factor ; Maturation-Promoting Factor - physiology ; meiosis ; Meiosis - genetics ; Meiosis - physiology ; Microinjections ; Microscopy, Electron ; Mutation ; Nuclear Envelope - chemistry ; Nuclear Envelope - ultrastructure ; nuclear pore complex ; nucleocytoplasmic transport ; Oncogene Proteins v-erbA - analysis ; Oncogene Proteins v-erbA - pharmacology ; Oncogene Proteins v-erbA - physiology ; Oocytes - physiology ; Oocytes - ultrastructure ; Receptors, Thyroid Hormone - antagonists &amp; inhibitors ; Receptors, Thyroid Hormone - genetics ; thyroid hormone receptor ; Xenopus laevis</subject><ispartof>Journal of cellular biochemistry, 1997-11, Vol.67 (2), p.184-200</ispartof><rights>Copyright © 1997 Wiley‐Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9328824$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nagl, Sylvia B.</creatorcontrib><creatorcontrib>Bunn, Caroline F.</creatorcontrib><creatorcontrib>Allison, Lizabeth A.</creatorcontrib><title>v-erbA oncogene initiates ultrastructural changes characteristic of early and intermediate events of meiotic maturation in Xenopus oocytes</title><title>Journal of cellular biochemistry</title><addtitle>J. Cell. Biochem</addtitle><description>The growth‐promoting properties of the retroviral v‐erbA oncogene, a highly mutated version of the chicken thyroid hormone receptor (TR) α, have so far exclusively been linked to dominant repression of the antimitogenic roles of TR and retinoic acid receptors. Here we show that when expressed in Xenopus oocytes v‐ErbA induced ultrastructural changes characteristic of early and intermediate events of meiotic maturation by activating gene transcription. v‐ErbA–induced maturation events occurred without activation of the cAMP/maturation‐promoting factor signal pathway and were arrested prior to meiotic spindle formation. The effects of v‐ErbA were not mimicked by a dominant negative in vitro–generated mutant of human TR, suggesting that v‐ErbA can contribute to cell cycle reentry by interference with regulatory pathways distinct from those involving TR. 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source Wiley-Blackwell Read & Publish Collection
subjects Animals
Biological Transport
Cell Nucleus - metabolism
Cell Nucleus - ultrastructure
Cyclic AMP - physiology
Cytoplasm - metabolism
Female
Gene Expression
Gene Transfer Techniques
Genes, erbA
Humans
maturation-promoting factor
Maturation-Promoting Factor - physiology
meiosis
Meiosis - genetics
Meiosis - physiology
Microinjections
Microscopy, Electron
Mutation
Nuclear Envelope - chemistry
Nuclear Envelope - ultrastructure
nuclear pore complex
nucleocytoplasmic transport
Oncogene Proteins v-erbA - analysis
Oncogene Proteins v-erbA - pharmacology
Oncogene Proteins v-erbA - physiology
Oocytes - physiology
Oocytes - ultrastructure
Receptors, Thyroid Hormone - antagonists & inhibitors
Receptors, Thyroid Hormone - genetics
thyroid hormone receptor
Xenopus laevis
title v-erbA oncogene initiates ultrastructural changes characteristic of early and intermediate events of meiotic maturation in Xenopus oocytes
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