Automated On-Line Sample Pretreatment System for the Determination of Trace Metals in Biological Samples by Inductively Coupled Plasma Mass Spectrometry

A fully automated on-line sample pretreatment system combining microwave digestion with sample preconcentration/matrix separation for the determination of trace metals (Fe, Ni, Cu, Zn, and Pb) in blood and serum samples by inductively coupled plasma mass spectrometry (ICPMS) was designed and evaluat...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1997-10, Vol.69 (19), p.3930-3939
Main Authors: Huang, Chuan-Chieh, Yang, Mo-Hsiung, Shih, Tung-Sheng
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Automation
Biological and medical sciences
Blood
Cattle
Chemistry
Fundamental and applied biological sciences. Psychology
Humans
Inorganic compounds
Mass Spectrometry - instrumentation
Mass Spectrometry - methods
Metals
Metals - blood
Other biological molecules
Plasma
Scientific imaging
Specimen Handling - methods
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Summary:A fully automated on-line sample pretreatment system combining microwave digestion with sample preconcentration/matrix separation for the determination of trace metals (Fe, Ni, Cu, Zn, and Pb) in blood and serum samples by inductively coupled plasma mass spectrometry (ICPMS) was designed and evaluated. The samples were mixed with an appropriate reagent and digested in a flow-through, focused microwave-heated oven. After digestion, the sample solution was transferred on-line to a column packed with iminodiacetate-based resin for separation of matrix elements like Na, K, Ca, Mg, Cl, P, and S that might interfere with the measurement by ICPMS. The analytes chelated on the resin were subsequently eluted and led to ICPMS for multielement determination. The whole sample pretreatment process was automatically controlled by a self-designed expert system. The analytical reliability of data from this on-line system was confirmed to be good with the analysis of SRM samples (Seronorm Whole Blood and NIST SRM 1598 Bovine Serum), and the limits of detection (3σ) for Fe, Ni, Cu, Zn, and Pb were 68, 0.34, 3.5, 13.4, and 0.22 μg/L, respectively. With this fully automated on-line system, the determination of analytes in biological fluid samples down to micrograms-per-liter levels has been proven to be feasible, and the sample throughput can achieve up to 6 samples/h.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac970284e