Major histocompatibility complex class II antigen expression in rejecting cardiac allografts : Detection using in vivo imaging with Radiolabeled monoclonal antibody

• Published in: Circulation (New York, N.Y.) N.Y.), 1997-09, Vol.96 (5), p.1605-1611
• Main Authors: MCGHIE, A. I, RADOVANCEVIC, B, CAPEK, P, MOORE, W. H, KASI, L, LAMKI, L, CLUBB, F. J, FRAZIER, O. H, WILLERSON, J. T
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal; Biological and medical sciences; Dogs; Graft Rejection - immunology; Graft Rejection - pathology; Graft Rejection - physiopathology; Heart Transplantation - immunology; Histocompatibility Antigens Class II - analysis; Histocompatibility Antigens Class II - immunology; Indium Radioisotopes; Medical sciences; Severity of Illness Index; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the heart; Transplantation, Homologous
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/01.CIR.96.5.1605

Increased expression of major histocompatibility complex class II (MHC-II) antigen occurs during cardiac allograft rejection. We tested the hypotheses that (1) radiolabeled antibody to MHC-II antigen allows detection of cardiac allograft rejection using nuclear imaging techniques and (2) uptake of radiolabeled antibody to MHC-II antigen correlates with severity of rejection. Thirteen beagles with cervical cardiac allografts were studied for 64+/-23 days by use of myocardial biopsy and in vivo imaging. Uptake of radiolabeled (131I [n=2], 123I [n=1], or 111In [n=10]) antibody to MHC-II increased over baseline in 7 animals that developed histological evidence of progressively worsening allograft rejection (group A), from 72.2+/-46.1 to 176.8+/-102.0 counts/pixel/mCi (P