Evidence for a direct interaction of superoxide anion radical with carnosine

Using photochemiluminescence, the interaction between carnosine and superoxide anion was measured directly. Carnosine at physiological concentrations decreased the amplitude of luminol chemiluminescence like superoxide dismutase (SOD) did, and prolonged the lag‐period of the chemiluminescence simila...

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Bibliographic Details
Published in:Biochemistry and molecular biology international 1997-09, Vol.43 (1), p.99-106
Main Authors: Klebanov, G. I., Teselkin, Yu.O., Babenkova, I. V., Popov, I. N., Levin, G., Tyulina, O. V., Boldyrev, A. A., Vladimirov, Yu.A.
Format: Article
Language:English
Subjects:
Antioxidant effect
Carnosine
Carnosine - metabolism
Carnosine - pharmacology
Luminescent Measurements
Luminol - metabolism
Nitroblue Tetrazolium - metabolism
Oxidation-Reduction
Photochemiluminescence
SOD
Superoxide radical
Superoxides - metabolism
Xanthine Oxidase - metabolism
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Summary:Using photochemiluminescence, the interaction between carnosine and superoxide anion was measured directly. Carnosine at physiological concentrations decreased the amplitude of luminol chemiluminescence like superoxide dismutase (SOD) did, and prolonged the lag‐period of the chemiluminescence similar to the effect of ascorbic acid. From the interaction of nitro blue tetrazolium with superoxide anion generated by the xanthine oxidase system, the constant for interaction of carnosine with O2* was calculated to be 105 M‐1. sec‐1. The possible biological significance of the quenching of superoxide anion by carnosine is discussed.
ISSN:1521-6543
1039-9712
1521-6551
DOI:10.1080/15216549700203861