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Transcription initiation sites and promoter structure of the mouse type 2 inositol 1,4,5-trisphosphate receptor gene
Transcription initiation sites and the promoter sequence of the ubiquitously expressed mouse type 2 inositol 1,4,5-trisphosphate receptor ( IP 3R2 ) gene were determined. In contrast to the nervous system-enriched IP 3R1 , the IP 3R2 gene had multiple (seven major) transcription initiation sites loc...
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Published in: | Gene 1997-09, Vol.196 (1), p.181-185 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Transcription initiation sites and the promoter sequence of the ubiquitously expressed mouse type 2 inositol 1,4,5-trisphosphate receptor (
IP
3R2
) gene were determined. In contrast to the nervous system-enriched
IP
3R1
, the
IP
3R2
gene had multiple (seven major) transcription initiation sites located 334 to 269
bp upstream from the first ATG codon. Transient luciferase assay revealed promoter activity of the
IP
3R2
sequence upstream from the transcription initiation sites. The
IP
3R2
promoter was GC-rich and had no conventional TATA box, but had a GC box in the proximal promoter. Multiple transcription start sites were flanked by CpG islands, and various
cis elements were located in the promoter. These structural features are considered to be responsible for a profile of
IP
3R2
gene expression. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/S0378-1119(97)00224-2 |