Detection and Typing of Human Papillomaviruses by Polymerase Chain Reaction in Cervical Scrapes of Croatian Women with Abnormal Cytology

The association between certain human papilomaviruses (HPV) and cervical intraepithelial neoplasia (CIN) is well documented, but still unknown among Croatian women. In 1995, women between the age of 17 and 64 with cytomorphologically abnormal smears (CIN I-IV) were tested for the presence of HPV. Co...

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Bibliographic Details
Published in:European journal of epidemiology 1997-09, Vol.13 (6), p.645-651
Main Authors: Grce, Magdalena, Husnjak, Koraljka, Magdić, Lada, Ilijaš, Marijan, Zlački, Marijan, Lepušić, Dubravko, Lukač, Josip, Hodek, Branko, Grizelj, Veselko, Kurjak, Asim, Kusić, Zvonko, Pavelić, Krešimir
Format: Article
Language:English
Subjects:
Adolescent
Adult
Age Factors
Age groups
Biological and medical sciences
Cell lines
Cellular biology
Cervical cancer
Cervical intraepithelial neoplasia
Cervical Intraepithelial Neoplasia - epidemiology
Cervical Intraepithelial Neoplasia - virology
Chi-Square Distribution
Croatia - epidemiology
Cytology
DNA
DNA Primers
Epidemiology
Female
Female genital diseases
Genomes
Gynecology
Gynecology. Andrology. Obstetrics
Human papillomavirus
Humans
Hybridization
Infections
Lesions
Medical sciences
Middle Aged
Neoplasia
Obstetrics
Pap smear
Papillomaviridae - genetics
Papillomaviridae - isolation & purification
Papillomavirus Infections - diagnosis
Papillomavirus Infections - epidemiology
Polymerase chain reaction
Polymerase Chain Reaction - methods
Prevalence
Risk Factors
Tumor Virus Infections - diagnosis
Tumor Virus Infections - epidemiology
Tumors
Vaginal Smears
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Summary:The association between certain human papilomaviruses (HPV) and cervical intraepithelial neoplasia (CIN) is well documented, but still unknown among Croatian women. In 1995, women between the age of 17 and 64 with cytomorphologically abnormal smears (CIN I-IV) were tested for the presence of HPV. Consensus and specific primers were used in the polymerase chain reaction (PCR) to detect the most common types: 6, 11, 16, 18, 31 and 33, as well as the unknown-risk HPV types (HPV X). Out of 379 specimens, 163 (43%) contained one or more HPV types. Coinfection with different HPV types in the same sample was observed in 16 cases. Beside low-risk HPV 6/11 (25.8%) the most frequently observed types were high-risk HPV types 16 (20.2%) and 31 (17.8%). Globally, the HPV positivity rate declines with age. The presence of HPV DNA significantly increased from 35.5 to 61.1% along with the severity of the cervical intraepithelial neoplasia (CIN I-IV). HPV type 6/11 was strongly associated with CIN I (33.8%), HPV type 31 with CIN II (22.9%), and HPV type 16 with CIN III (50%).
ISSN:0393-2990
1573-7284
DOI:10.1023/A:1007323405069