Detection of BK Polyomavirus Genotypes in Healthy and HIV-Positive Children

Urine samples from 211 community children (3-7 years age), from 33 HIV type-1 infected children and from 56 HIV-negative children were collected and analyzed for the presence of BK virus (BKV) DNA by PCR. PCR amplifications were carried out using primers specific for the BKV structural region VP1. W...

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Bibliographic Details
Published in:European journal of epidemiology 1997-09, Vol.13 (6), p.653-657
Main Authors: Di Taranto, C., Pietropaolo, V., Orsi, G. B., Jin, L., Sinibaldi, L., Degener, A. M.
Format: Article
Language:English
Subjects:
Age groups
AIDS/HIV
Biological and medical sciences
BK virus
BK Virus - isolation & purification
Bone marrow
Case-Control Studies
Child
Child, Preschool
Children
Deoxyribonucleic acid
DNA
DNA, Viral - urine
Female
Genotype
Genotypes
Health care
HIV
HIV 1
HIV Seronegativity
HIV Seropositivity - immunology
HIV Seropositivity - virology
Hospitalization
Human immunodeficiency virus
Humans
Immunocompromised Host
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Infections
Italy - epidemiology
Male
Medical sciences
Polymerase Chain Reaction
Polymorphism
Polymorphism, Restriction Fragment Length
Polyomavirus Infections - epidemiology
Polyomavirus Infections - urine
Prevalence
Statistics, Nonparametric
Transplants & implants
Tumor Virus Infections - epidemiology
Tumor Virus Infections - urine
Urine
Viruses
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Summary:Urine samples from 211 community children (3-7 years age), from 33 HIV type-1 infected children and from 56 HIV-negative children were collected and analyzed for the presence of BK virus (BKV) DNA by PCR. PCR amplifications were carried out using primers specific for the BKV structural region VP1. We also investigated the distribution of BKV subtypes by a restriction fragment polymorphism assay (RFLP). We demonstrated BKV DNA in 3.8% of 211 community children with a higher prevalence of subtype I. In HIV-1 positive children we detected BKV DNA in 2 urine samples (6%) out of 33, both belonging to subtype I. The HIV-negative cluster did not show any positivity to BKV DNA. The results confirm a more frequent primary BKV infection in children of 3-5 years of age and a higher prevalence in hospitalized children affected by HIV-1. The most relevant finding was that among both the community and HIV-1 positive children the subtype I was the most frequently detected.
ISSN:0393-2990
1573-7284
DOI:10.1023/A:1007371320999