The Effect of Ashing on Cells: Spectromicroscopy of Physiological Elements

We analyzed the effects of cold oxygen plasma ashing of neurobiological specimens on different elements with synchrotron spectromicroscopy. Our results demonstrate that while carbon is almost completely removed, phosphorus, calcium, potassium, sulfur, and, to some extent, nitrogen are retained and t...

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Bibliographic Details
Published in:Analytical biochemistry 1997-10, Vol.252 (1), p.106-109
Main Authors: De Stasio, Gelsomina, Capozi, M., Droubay, T.C., Mercanti, D., Ciotti, M.T., Lorusso, G.F., Andres, R., Suda, T., Perfetti, P., Tonner, B.P., Margaritondo, G.
Format: Article
Language:English
Subjects:
Animals
Calcium - chemistry
Carbon - chemistry
Cerebellum - ultrastructure
Microscopy, Electron - methods
Nitrogen - chemistry
Oxygen - chemistry
Phosphorus - chemistry
Potassium - chemistry
Rats
Spectrometry, X-Ray Emission - methods
Sulfur - chemistry
Tissue Fixation - methods
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Summary:We analyzed the effects of cold oxygen plasma ashing of neurobiological specimens on different elements with synchrotron spectromicroscopy. Our results demonstrate that while carbon is almost completely removed, phosphorus, calcium, potassium, sulfur, and, to some extent, nitrogen are retained and their relative concentration is enhanced.
ISSN:0003-2697
1096-0309
DOI:10.1006/abio.1997.2276