Restriction Fragment Length Polymorphism Analysis and Random Amplified Polymorphic DNA Analysis of Campylobacter jejuni Strains Isolated from Patients with Guillain-Barré Syndrome

Campylobacter jejuni serotype O19 strains associated with the Guillain-Barré syndrome (GBS) and other strains were examined by restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction products of the flaA genes and by random amplified polymorphic DNA (RAPD) analysis. RFL...

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Bibliographic Details
Published in:The Journal of infectious diseases 1997-10, Vol.176 (4), p.1105-1108
Main Authors: Fujimoto, Shuji, Allos, Ban Mishu, Misawa, Naoaki, Patton, Charlotte M., Blaser, Martin J.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Bacterial - analysis
Bacterial diseases
Bacterial diseases of the nervous system. Bacterial myositis
Biological and medical sciences
Campylobacter
Campylobacter Infections - genetics
Campylobacter Infections - immunology
Campylobacter jejuni
Campylobacter jejuni - genetics
Campylobacter jejuni - immunology
Concise Communications
DNA
DNA, Bacterial - analysis
Flagellin - genetics
Genetic polymorphism
Guillain Barre syndrome
Human bacterial diseases
Humans
Infections
Infectious diseases
Medical sciences
Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis
Neurology
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Polyradiculoneuropathy - microbiology
Poultry - microbiology
Random Amplified Polymorphic DNA Technique
Restriction fragment length polymorphism
Serotyping
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Summary:Campylobacter jejuni serotype O19 strains associated with the Guillain-Barré syndrome (GBS) and other strains were examined by restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction products of the flaA genes and by random amplified polymorphic DNA (RAPD) analysis. RFLP analysis showed that regardless of LIO serotype, geographic origins, or association with GBS, the O19 isolates shared an identical digestion pattern by each of four restriction endonucleases, DdeI, MboI, MseI, and AluI. In contrast, among C. jejuni O1 or O2 strains, RFLP patterns were different even among strains of the same LIO serotype. The results of the RAPD analysis were consistent with the flaA RFLP data. These data indicate that all of the O19 strains that were tested were closely related to one another whether they were or were not associated with GBS.
ISSN:0022-1899
1537-6613
DOI:10.1086/516522