cDNA Cloning of a Short Type of Multidrug Resistance Protein Homologue,SMRP,from a Human Lung Cancer Cell Line

Members of the ATP binding cassette (ABC) superfamily are involved in the energy-dependent transport of a wide variety of substrates including anticancer agents across the membranes. We have cloned a cDNA fragment including a novel ABC sequence from a cisplatin-resistant human lung adenocarcinoma ce...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1997-09, Vol.238 (3), p.790-794
Main Authors: Suzuki, Toshihiro, Nishio, Kazuto, Sasaki, Hiroki, Kurokawa, Hirokazu, Saito-Ohara, Fumiko, Ikeuchi, Tatsuro, Tanabe, Shinzo, Terada, Masaaki, Saijo, Nagahiro
Format: Article
Language:English
Subjects:
Amino Acid Sequence
ATP-Binding Cassette Transporters - genetics
ATP-Binding Cassette Transporters - isolation & purification
Base Sequence
Carcinoma, Non-Small-Cell Lung - chemistry
Carcinoma, Non-Small-Cell Lung - genetics
Chromosome Mapping
Chromosomes, Human, Pair 3
Cisplatin - pharmacology
Cloning, Molecular
DNA, Complementary - isolation & purification
Drug Resistance, Multiple
Humans
Lung Neoplasms - chemistry
Lung Neoplasms - genetics
Molecular Sequence Data
Multidrug Resistance-Associated Proteins
Multigene Family
Neoplasm Proteins - genetics
Neoplasm Proteins - isolation & purification
Polymerase Chain Reaction
Tumor Cells, Cultured
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Summary:Members of the ATP binding cassette (ABC) superfamily are involved in the energy-dependent transport of a wide variety of substrates including anticancer agents across the membranes. We have cloned a cDNA fragment including a novel ABC sequence from a cisplatin-resistant human lung adenocarcinoma cell line, PC-14/CDDP, by reverse transcription polymerase chain reaction (RT-PCR) using degenerate primers and screened a cDNA library using the cDNA fragment as a probe. A full-length cDNA clone, BM4.8, was obtained. Sequence analysis showed that the cDNA encoded a short type of multidrug resistance protein homologue,SMRP,by computed homology search.SMRPwas composed of 946 amino acids and had two ABCs with walker A and B motifs. This gene was mapped on chromosome 3 at band q27 by fluorescencein situhybridization (FISH) analysis and was found to be expressed in various tissues by Northern blot analysis.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1997.7346