cDNA Cloning of a Short Type of Multidrug Resistance Protein Homologue,SMRP,from a Human Lung Cancer Cell Line

• Published in: Biochemical and biophysical research communications 1997-09, Vol.238 (3), p.790-794
• Main Authors: Suzuki, Toshihiro, Nishio, Kazuto, Sasaki, Hiroki, Kurokawa, Hirokazu, Saito-Ohara, Fumiko, Ikeuchi, Tatsuro, Tanabe, Shinzo, Terada, Masaaki, Saijo, Nagahiro
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; ATP-Binding Cassette Transporters - genetics; ATP-Binding Cassette Transporters - isolation & purification; Base Sequence; Carcinoma, Non-Small-Cell Lung - chemistry; Carcinoma, Non-Small-Cell Lung - genetics; Chromosome Mapping; Chromosomes, Human, Pair 3; Cisplatin - pharmacology; Cloning, Molecular; DNA, Complementary - isolation & purification; Drug Resistance, Multiple; Humans; Lung Neoplasms - chemistry; Lung Neoplasms - genetics; Molecular Sequence Data; Multidrug Resistance-Associated Proteins; Multigene Family; Neoplasm Proteins - genetics; Neoplasm Proteins - isolation & purification; Polymerase Chain Reaction; Tumor Cells, Cultured
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1997.7346

Members of the ATP binding cassette (ABC) superfamily are involved in the energy-dependent transport of a wide variety of substrates including anticancer agents across the membranes. We have cloned a cDNA fragment including a novel ABC sequence from a cisplatin-resistant human lung adenocarcinoma cell line, PC-14/CDDP, by reverse transcription polymerase chain reaction (RT-PCR) using degenerate primers and screened a cDNA library using the cDNA fragment as a probe. A full-length cDNA clone, BM4.8, was obtained. Sequence analysis showed that the cDNA encoded a short type of multidrug resistance protein homologue,SMRP,by computed homology search.SMRPwas composed of 946 amino acids and had two ABCs with walker A and B motifs. This gene was mapped on chromosome 3 at band q27 by fluorescencein situhybridization (FISH) analysis and was found to be expressed in various tissues by Northern blot analysis.