A Global Gene Pool for High-Level Cephalosporin Resistance in Commensal Streptococcus Species and Streptococcus Pneumoniae

Highly penicillin- and cephalosporin-resistant Streptococcus mitis and Streptococcus oralis were isolated in Spain, Hungary, and Berlin. With chromosomal DNA of these strains, resistant transformants of Streptococcus pneumoniae were obtained that expressed low-affinity variants of penicillin- bindin...

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Bibliographic Details
Published in:The Journal of infectious diseases 1997-10, Vol.176 (4), p.1001-1012
Main Authors: Reichmann, Peter, König, Andrea, Linares, Josefina, Alcaide, Fernando, Tenover, Fred C., Swidsinski, Sonja, Hakenbeck, Regine
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Antibiotics
Bacteriology
Base Sequence
Berlin - epidemiology
Biological and medical sciences
Carrier Proteins - genetics
Carrier Proteins - metabolism
Cefotaxime - pharmacology
Cephalosporin Resistance - genetics
Cephalosporins - pharmacology
DNA
DNA, Bacterial - analysis
DNA, Bacterial - genetics
Drug Resistance, Microbial - genetics
Epidemiology
Fundamental and applied biological sciences. Psychology
Gene Expression
Gene Transfer Techniques
Genes
Genes, Bacterial
Genetic mutation
Humans
Hungary - epidemiology
Microbial Sensitivity Tests
Microbiology
Molecular Epidemiology
Molecular Sequence Data
Mosaic
Penicillin
Penicillin Resistance - genetics
Penicillin-Binding Proteins
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
Spain - epidemiology
Streptococcal Infections - drug therapy
Streptococcal Infections - epidemiology
Streptococcal Infections - genetics
Streptococcus - genetics
Streptococcus mitis
Streptococcus oralis
Streptococcus oralis - genetics
Streptococcus pneumoniae
Streptococcus pneumoniae - genetics
Transformation, Genetic
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Description
Summary:Highly penicillin- and cephalosporin-resistant Streptococcus mitis and Streptococcus oralis were isolated in Spain, Hungary, and Berlin. With chromosomal DNA of these strains, resistant transformants of Streptococcus pneumoniae were obtained that expressed low-affinity variants of penicillin- binding proteins (PBPs) 2x, 1a, 2a, and 2b in different combinations, depending on the selective conditions. The transformants had cefotaxime MICs of up to 6 µg/mL, and those with a low-affinity PBP 2b were highly deficient in penicillin-induced lysis. Sequence analysis of the pbp2x genes confirmed the presence of a global gene pool of penicillin resistance determinants shared by commensal and pathogenic streptococci.
ISSN:0022-1899
1537-6613
DOI:10.1086/516532