Oocyte retrieval and histological studies of follicular population in buffalo ovaries

Ovaries ( N = 250) from slaughtered buffaloes were collected to study follicular population and compare methods of oocyte retrieval. The number and size of surface follicles were recorded and grouped into different categories. Different sized follicles in relation to oocyte diameter were studied his...

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Bibliographic Details
Published in:Animal reproduction science 1997-06, Vol.47 (3), p.189-195
Main Authors: Kumar, A., Solanki, V.S., Jindal, S.K., Tripathi, V.N., Jain, G.C.
Format: Article
Language:English
Subjects:
Animals
BUFALO DE AGUA
Buffaloes - physiology
BUFFLE D'EAU
DIAMETER
DIAMETRE
DIAMETRO
Female
Fertilization in Vitro - methods
Fertilization in Vitro - veterinary
FOLICULOS OVARICOS
FOLLICULE OVARIEN
Oocytes - cytology
Oocytes - physiology
OVA
OVAIRE
Ovarian Follicle - cytology
Ovarian Follicle - physiology
OVARIAN FOLLICLES
OVARIES
OVARIOS
Ovary - cytology
Ovary - physiology
OVULE
OVULO
WATER BUFFALOES
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Summary:Ovaries ( N = 250) from slaughtered buffaloes were collected to study follicular population and compare methods of oocyte retrieval. The number and size of surface follicles were recorded and grouped into different categories. Different sized follicles in relation to oocyte diameter were studied histologically. Yield of oocytes per ovary were less ( P < 0.05) from ovaries bearing a corpus luteum (CL). Techniques used for oocyte recovery included slicing, follicle puncture and aspiration. The oocyte recovery rate was greatest ( P < 0.05) using slicing. The average number of visible surface follicles was 5.20 ± 0.97 with mean numbers of 2.5, 1.2, 0.82 and 0.62 per ovary for follicles sized 4, 8, 12 and 12 mm respectively. Histological studies revealed large numbers of primordial follicles in prepubertal and atretic follicles in senile buffaloes. They also established a biphasic relationship of growth between oocyte diameter and follicular size.
ISSN:0378-4320
1873-2232
DOI:10.1016/S0378-4320(96)01588-6