A Synthetic Linear Decapeptide Binds to the Atrial Natriuretic Peptide Receptors and Demonstrates Cyclase Activation and Vasorelaxant Activity

• Published in: The Journal of biological chemistry 1989-12, Vol.264 (34), p.20309-20313
• Main Authors: Bovy, P.R., O'Neal, J.M., Olins, G.M., Patton, D.R., Mehta, P.P., McMahon, E.G., Palomo, M, Schuh, J, Blehm, D
• Format: Article
• Language: English
• Subjects: Adenylyl Cyclases - metabolism; Amino Acid Sequence; Animals; aorta; Aorta - drug effects; Aorta - physiology; Atrial Natriuretic Factor - chemical synthesis; Atrial Natriuretic Factor - metabolism; Atrial Natriuretic Factor - pharmacology; atrial natriuretic hormone; Biological and medical sciences; Cell Membrane - metabolism; Cell receptors; Cell structures and functions; Enzyme Activation; Fundamental and applied biological sciences. Psychology; In Vitro Techniques; Indicators and Reagents; Kinetics; Lung - metabolism; Molecular and cellular biology; Molecular Sequence Data; Muscle, Smooth, Vascular - drug effects; Muscle, Smooth, Vascular - physiology; Oligopeptides - chemical synthesis; Oligopeptides - metabolism; Oligopeptides - pharmacology; Rabbits; Receptors, Atrial Natriuretic Factor; Receptors, Cell Surface - metabolism; Vasodilation - drug effects
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/S0021-9258(19)47063-4

A linear decapeptide, [cyclohexylalanine 106]ANP-(105-114)NH2 (1), where ANP is atrial natriuretic peptide, was prepared by solid phase synthesis and purified by reverse-phase liquid chromatography. This novel peptide was found to bind to ANP receptors in rabbit lung membranes, to stimulate cGMP production in various tissues, and to fully relax precontracted rabbit aorta in a dose-dependent fashion. The potency of 1 in the various in vitro assays varies between one-twentieth and one-eightieth of the potency of the reference peptide, the 24-mer rat ANP-(103-126). The linear decapeptide 1, which encompasses amino acid residues from the rat ANP sequence (105-114), features a cyclohexylalanine residue instead of the phenylalanine 106 residue in the hormone sequence, a free sulfhydryl function at the N-terminal cysteine 105, and a carboxamide C terminus. Its disulfide dimer 6 was active in the rabbit aorta assay while the S-methyl cysteine 7 analogue was not active in the same assay at similar concentrations. The decapeptide 1 is of particular significance because it is the shortest analogue reported to date endowed with agonistic activity at the guanylate cyclase-coupled ANP receptor. In particular, it is interesting to compare its structure to the structures of other short linear analogues of ANP which are totally devoid of the ability to stimulate particulate guanylate cyclase activity.