Molecular cloning and expression of a rat cDNA encoding 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase

The cDNA of a 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase) was isolated from rat liver RNA by reverse transcription and the polymerase chain reaction (PCR). The rat AICARFT/IMPCHase cDNA included 1928 bp containing a coding region of 1779 bp f...

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Bibliographic Details
Published in:Gene 1997-09, Vol.197 (1), p.289-293
Main Authors: Akira, Toko, Komatsu, Masami, Nango, Rieko, Tomooka, Akiko, Kimiko Konaka, Yamauchi, Masatake, Kitamura, Yukihiko, Nomura, Shintaro, Tsukamoto, Ikuyo
Format: Article
Language:English
Subjects:
10-Formyltetrahydrofolate
Amino Acid Sequence
Animals
Bifunctional protein
Cloning, Molecular
DNA, Complementary - genetics
Escherichia coli - genetics
Gene Expression Regulation, Enzymologic - genetics
Hepatectomy
Hydroxymethyl and Formyl Transferases - genetics
Liver - physiology
Liver Regeneration - genetics
Molecular Sequence Data
Nucleotide Deaminases - genetics
Phosphoribosylaminoimidazolecarboxamide Formyltransferase
purH gene
Purine synthesis
Rats
Recombinant Proteins
RNA, Messenger - analysis
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sequence Homology, Nucleic Acid
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Summary:The cDNA of a 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase (AICARFT/IMPCHase) was isolated from rat liver RNA by reverse transcription and the polymerase chain reaction (PCR). The rat AICARFT/IMPCHase cDNA included 1928 bp containing a coding region of 1779 bp for a 592-amino acid polypeptide ( M r=64 200). Rat and human AICARFT/IMPCHase cDNAs show 84 and 91% homology at the nucleotide and amino acid sequence level, respectively. The protein produced by the rat cDNA using pET-expression system catalysed the penultimate and final steps of de novo purine biosynthesis. Northern analysis identified a 2.8-kb AICARFT/IMPCHase mRNA and the level of the AICARFT/IMPCHase transcripts increased markedly at 24 h after partial (70%) hepatectomy.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(97)00273-4