Nucleotide Sequences of 5' and 3' Non-coding Regions of Pepper Mild Mottle Virus Strain S RNA

U.E.I. Fitopatología, Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006 Madrid, Spain The nucleotide sequences of the 5' and 3' non-coding regions of pepper mild mottle virus strain S (PMMV-S) RNA were determined; they are more like corresponding sequences of tomato mosaic v...

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Bibliographic Details
Published in:Journal of general virology 1989-11, Vol.70 (11), p.3025-3031
Main Authors: Avila-Rincon, M. J, Ferrero, M. L, Alonso, E, Garcia-Luque, I, Diaz-Ruiz, J. R
Format: Article
Language:English
Subjects:
Base Sequence
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Genes, Viral
Genetics
Hydrogen Bonding
Microbiology
Molecular Sequence Data
Molecular Weight
Nucleic Acid Conformation
Plant Viruses
Protein Biosynthesis
RNA, Viral - genetics
Vegetables
Viral Proteins - genetics
Viral Structural Proteins - genetics
Virology
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Summary:U.E.I. Fitopatología, Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006 Madrid, Spain The nucleotide sequences of the 5' and 3' non-coding regions of pepper mild mottle virus strain S (PMMV-S) RNA were determined; they are more like corresponding sequences of tomato mosaic virus (ToMV) RNA than those of any other tobamovirus reported so far. The 5' leader contains a 68 nucleotide guanosine-free sequence which differs in several nucleotides from the corresponding sequences in genomic RNA of tobacco mosaic virus (TMV) and ToMV. The messenger activity of PMMV-S RNA in vitro and the polypeptide translation products made were similar to those of TMV RNA. It therefore seems unlikely that qualitative or quantitative differences in translation in vivo account for the milder symptoms induced by PMMV-S, and its lesser replication, than TMV. The 3' non-coding region of PMMV-S RNA is 199 nucleotides long and can be folded into the same secondary structure as the RNA of other tobamoviruses. Keywords: PMMV-S, non-coding terminal sequences, RNA translation, tobamovirus Received 3 May 1989; accepted 13 July 1989.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-70-11-3025