Effects of dietary pantethine levels on drug-metabolizing system in the liver of rats orally administered varying amounts of autoxidized linoleate

• Published in: Journal of Nutritional Science and Vitaminology 1989, Vol.35(4), pp.303-313
• Main Authors: Hiramatsu, N. (Himeji Junior Coll., Hyogo (Japan)), Kishida, T, Natake, M
• Format: Article
• Language: English
• Subjects: ACIDE LINOLEIQUE; ACIDE PANTOTHENIQUE; ACIDO LINOLEICO; ACIDO PANTOTENICO; aminopyrin-N-demethylase; Animals; autoxidized linoleic acid; cytochrome b5; cytochrome P-450; Cytochrome P-450 Enzyme System - metabolism; Cytochromes b5 - metabolism; CYTOPLASMIC ORGANELLES; Diet; Dose-Response Relationship, Drug; drug-metabolizing activity; electron transfer system; Electron Transport - drug effects; ENZIMAS; ENZYME; ENZYMES; FOIE; Growth - drug effects; HIGADO; LINOLEIC ACID; Linoleic Acids - administration & dosage; Linoleic Acids - pharmacology; LIVER; Liver - drug effects; Liver - metabolism; Male; METABOLISM; METABOLISME; METABOLISMO; microsome; Microsomes, Liver - drug effects; Mixed Function Oxygenases - metabolism; ORGANITE CELLULAIRE; ORGANULOS CITOPLASMATICOS; Oxidation-Reduction; Pantetheine - administration & dosage; Pantetheine - pharmacology; pantethine; PANTOTHENIC ACID; RAT; RATA; RATS; Rats, Inbred Strains; S-9 activity; Sulfhydryl Compounds - pharmacology
• ISSN: 0301-4800; 1881-7742
• DOI: 10.3177/jnsv.35.303

The effects of dietary pantethine levels on the drugmetabolizing system were investigated under administration of varying amounts of autoxidized linoleate (AL) with rat liver microsomes and S-9 fractions. AL having 800meq/kg of peroxide value and 1, 700meq/kg of carbonyl value was dosed to the rats of each group given drinking water containing 0mg% (deficient), 6.25mg% (normal), and 125mg% pantethine (sufficient). The contents and activities of the enzymes in the drugmetabolizing system in the rat liver of each pantethine-level group changed essentially in a similar manner, that is, they were induced at an AL daily dose of 0.2ml/100g body weight (i.e., small dose) for 5 successive days and lowered at a daily dose of 0.4ml/100g body weight (i.e., large dose) by the same administration period, compared with respective nonAL groups in each of the three pantethine levels. In both non-AL and the small-dose AL, enzyme activities of the electron transfer system in rat liver microsomes, aminopyrin-N-demethylase activity, and metabolic activation of 2-acetylaminofluorene in S-9 fractions were significantly higher in the pantethine-deficient group than in the pantethine-normal and sufficient groups. In the large-dose AL, the enzyme activities in the drugmetabolizing system decreased significantly in any pantethine levels, though the survival rate of the rats was higher in the pantethine-sufficient group than in the pantethine-normal groups. The results suggest that the pantethine relieves the effect of dosed AL on the drug-metabolizing system in rat liver.