The BST1 gene of Saccharomyces cerevisiae is the sphingosine-1-phosphate lyase

Sphingolipids elicit a wide variety of eukaryotic cellular responses, most involving regulation of cell growth, differentiation, and apoptosis. Sphingosine 1-phosphate, a sphingolipid catabolite, is mitogenic in fibroblasts and inhibits the chemotactic mobility and invasiveness of human tumor cells....

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 1997-10, Vol.272 (42), p.26087-26090
Main Authors: Saba, J.D. (Children's Hospital Oakland Research Institute, Oakland, CA.), Nara, F, Bielawska, A, Garrett, S, Hannun, Y.A
Format: Article
Language:English
Subjects:
ALCOHOLS
Aldehyde-Lyases - genetics
Aldehyde-Lyases - metabolism
AMINO COMPOUNDS
CLONING
Cloning, Molecular
D-ERYTHRO-SPHINGOSINE
DELETIONS
ENZYMIC ACTIVITY
GENE EXPRESSION
GENES
Genes, Fungal
LYASES
Lysophospholipids
METABOLISM
MUTANTS
PHOSPHATES (ESTERS)
PHYTOSPINGOSINE
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Sphingosine - analogs & derivatives
Sphingosine - metabolism
STRUCTURAL GENES
UPTAKE
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sphingolipids elicit a wide variety of eukaryotic cellular responses, most involving regulation of cell growth, differentiation, and apoptosis. Sphingosine 1-phosphate, a sphingolipid catabolite, is mitogenic in fibroblasts and inhibits the chemotactic mobility and invasiveness of human tumor cells. Sphingosine 1-phosphate degradation requires cleavage at the C2-3 carbon bond by sphingosine phosphate lyase. A yeast genetic approach was used to clone the first sphingosine phosphate lyase gene, BST1. BST1 overexpression conferred resistance to sphingosine in yeast. BST1 deletion produced sensitivity to exogenous D-erythro-sphingosine and phytosphingosine and intracellular accumulation of sphingosine 1-phosphate upon exposure to exogenous sphingosine. This study confirms that sphingoid base metabolism is similar in all eukaryotes and suggests that yeast genetics may be useful in the isolation and identification of other genes involved in sphingolipid signaling and metabolism.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.272.42.26087