Sensitive bioaffinity assays with individual microparticles and time-resolved fluorometry

Future immunoassays and nucleic acid hybridization assays will be performed in miniaturized formats that utilize microchips or microparticles. This will require a sensitive detection technology that allows spatial resolution. By using fluorescent europium chelates and time-resolved microfluorometry,...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 1997-10, Vol.43 (10), p.1937-1943
Main Authors: Lovgren, Timo, Heinonen, Pia, Lehtinen, Paivi, Hakala, Harri, Heinola, Johanna, Harju, Raimo, Takalo, Harri, Mukkala, Veli-Matti, Schmid, Ruth, Lonnberg, Harri, Pettersson, Kim, Iitia, Antti
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chelating Agents
Cystic Fibrosis - genetics
Europium
Humans
Immunoassay - standards
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Miscellaneous. Technology
Mutation
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Prostate-Specific Antigen - analysis
Sensitivity and Specificity
Spectrometry, Fluorescence - methods
Time Factors
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Summary:Future immunoassays and nucleic acid hybridization assays will be performed in miniaturized formats that utilize microchips or microparticles. This will require a sensitive detection technology that allows spatial resolution. By using fluorescent europium chelates and time-resolved microfluorometry, one can detect 11,000 europium molecules on individual microparticles. In a miniaturized noncompetitive immunoassay of prostate-specific antigen (PSA), we quantitatively detected 5 ng/L (0.05 amol per particle) of the analyte on an individual microparticle with excellent precision over the whole measurement range (CV
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/43.10.1937